Figure 1: In vitro differentiation of iPSCs into iPSC-Heps. iPSCs were seeded at 2 × 104 cells/cm2, maintained in Dulbecco’s modified Eagle’s medium. (a) Left: Morphology of iPSC colonies. Right: iPSC colonies were positive for alkaline phosphate stain (purple). (b) The hepatogenic differentiation was induced by a 2-step procedure as described in Section 2. Morphology of undifferentiated and differentiated iPSCs was evaluated at different days after hepatogenic differentiation.