Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices

Figure 3

Comparison of the yield of low molecular weight protein/peptide extraction from serum by means of differential solubilization (DS), organic precipitation (OP), and ultrafiltration (UF). All techniques effectively remove the high molecular weight serum protein, whereas recovery of LMW proteins/peptides is highest with DS. Reprinted with permission from Kawashima et al. [94]. Copyright 2011 American Chemical Society.