Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices
Figure 4
Effect on the number of detected (neuro)peptides (from mouse hypothalamus) by postmortem time (time between tissue collection and heat denaturation/stabilization) [104]. (a) Number of detected peptides by nano-LC ESI MS rapidly increases with postmortem time. (b, c) Two-dimensional peptide displays of control group and 10 min postmortem group, respectively. Picture adapted from Sköld et al. [104].
