Bronchiolitis Obliterans Organizing Pneumonia in Swine Associated with Porcine Circovirus Type 2 Infection

Table 1

Sequence of primers used for these pathogens detection by PCR analysis.

Pathogen	Primer sequence
Porcine reproductive and respiratory syndrome virus (PRRSV)	Forward primer: 5^′-CCC GGG TTG AAA AGC CTC GTG T-3^′
	Reverse primer: 5^′- TGT AAC TTA TCC TCC CTG AAT CTG-3^′
Pseudorabies virus (PRV)	gD, forward primer: 5^′-CAC GGA AGA GAT GGG GCT-3^′
	Reverse primer: 5^′-GTC GAC GCC CGC TTG AAG CT-3^′
Classical swine fever virus (CSFV)	Forward primer: 5^′-TTA AAR ATA GCC CCA AAA GAG CAT G-3^′
	Reverse primer: 5^′-CTG GCG TCC ATC ATY CCG YGT AT-3^′
Porcine parvovirus (PPV)	VP2, forward primer: 5^′-GCA GTA CCA ATT CAT CTT CT-3^′
	Reverse primer: 5^′-TGG TCT CCT TCT GTG GTA GG-3^′
Cytomegalovirus (CMV)	gB, forward primer: 5^′-CCC TGA TCT TAA ATG ACG AGG ACG TGA C-3^′
	Reverse primer: 5^′-ACC GTC TGA GAG AGA CTG AAC TTC TCT GAC AC-3^′
Porcine enterovirus (PEV)	Talfan-3D, forward primer: 5^′-CAA AGA CTG GTC CTT CAT TG-3^′
	Reverse primer: 5^′-ATA CGC CGA GCG CGG AAG AT-3^′
Porcine circovirus type 1 (PCV1)	Forward primer: 5^′-ATA CGG TAG TAT TGG AAA GGT AGG G-3^′
	Reverse primer: 5^′-ACA CTC GAT AAG TAT GTG GCC TTC T-3^′
Mycoplasma hyopneumoniae	Forward primer: 5^′-GAG CCT TCA AGC TTC ACC AAG A-3^′
	Reverse primer: 5^′-TGT GTT AGT GAC TTT TGC CAC C-3^′
Porcine coronavirus	Forward primer: 5^′-GCC ATT GAT TTA TGG AGA CA-3^′
	Reverse primer: 5^′-GTA TAA AAC CTC CTG GCT GT-3^′
Swine influenza virus (SIV)	Forward primer: 5^′-AGT ATA CAG CCT AAT CAG AC-3^′
	Reverse primer: 5^′-AGT AGA AAC AAG GGT ATT TTT C-3^′
Porcine circovirus type 2 (PCV2)	ORF-2, forward primer: 5^′-GGT TTG TAG CCT CAG CCA AAG C-3^′
	Reverse primer: 5^′-GCA CCT TCG GAT ATA CTG TCA AGG-3^′