Table 1: Sequence of primers used for these pathogens detection by PCR analysis.

PathogenPrimer sequence

Porcine reproductive and respiratory syndrome virus (PRRSV)Forward primer: -CCC GGG TTG AAA AGC CTC GTG T-
Reverse primer: - TGT AAC TTA TCC TCC CTG AAT CTG-
Pseudorabies virus (PRV)gD, forward primer: -CAC GGA AGA GAT GGG GCT-
Reverse primer: -GTC GAC GCC CGC TTG AAG CT-
Classical swine fever virus (CSFV)Forward primer: -TTA AAR ATA GCC CCA AAA GAG CAT G-
Reverse primer: -CTG GCG TCC ATC ATY CCG YGT AT-
Porcine parvovirus (PPV)VP2, forward primer: -GCA GTA CCA ATT CAT CTT CT-
Reverse primer: -TGG TCT CCT TCT GTG GTA GG-
Cytomegalovirus (CMV)gB, forward primer: -CCC TGA TCT TAA ATG ACG AGG ACG TGA C-
Reverse primer: -ACC GTC TGA GAG AGA CTG AAC TTC TCT GAC AC-
Porcine enterovirus (PEV)Talfan-3D, forward primer: -CAA AGA CTG GTC CTT CAT TG-
Reverse primer: -ATA CGC CGA GCG CGG AAG AT-
Porcine circovirus type 1 (PCV1)Forward primer: -ATA CGG TAG TAT TGG AAA GGT AGG G-
Reverse primer: -ACA CTC GAT AAG TAT GTG GCC TTC T-
Mycoplasma hyopneumoniaeForward primer: -GAG CCT TCA AGC TTC ACC AAG A-
Reverse primer: -TGT GTT AGT GAC TTT TGC CAC C-
Porcine coronavirusForward primer: -GCC ATT GAT TTA TGG AGA CA-
Reverse primer: -GTA TAA AAC CTC CTG GCT GT-
Swine influenza virus (SIV)Forward primer: -AGT ATA CAG CCT AAT CAG AC-
Reverse primer: -AGT AGA AAC AAG GGT ATT TTT C-
Porcine circovirus type 2 (PCV2)ORF-2, forward primer: -GGT TTG TAG CCT CAG CCA AAG C-
Reverse primer: -GCA CCT TCG GAT ATA CTG TCA AGG-