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252141.fig.003b
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Figure 3: Immunohistochemical evaluation of aorta in unoperated as well as saline or elastase (28 day) perfused mice. Elastic van Gieson staining ((a), (b), (c)) demonstrated destruction of the medial elastin layer in elastase animals ((c), arrow). Masson's trichrome stain ((d), (e), (f)) shows enhanced deposition of extracellular matrix among SMCs in elastase-treated animals ((f), arrow). SM alpha-actin content, as denoted by red staining, is significantly reduced in injured vessels of original medial layer ((i), arrow) compared to Unoperated (g) and Sham controls (h). Black bars = 50 μm