Herpes Simplex Virus Type-1 (HSV-1) Entry into Human Mesenchymal Stem Cells Is Heavily Dependent on Heparan Sulfate
Figure 3
HSV-1 replicates in infected hMSCs. (a) Visualization and quantification of HSV-1 replication in cultured hMSCs. Confluent monolayers of hMSCs (5 × 106) were infected with HSV-1 KOS (804) virus at 0.01 PFU/cell were fixed and Giemsa stained at 0 hr (panel (A)) 12 hr (panel (B)) 24 hr (panel (C)) and 48 hr (panel (D)) postinfection. The numbers of plaques were visualized. (b) The number of plaques formed postinfection increased in a time-dependent manner. Error bars represent standard deviations. (), one way ANOVA. (c) Infectious yields of HSV-1 during viral infection were quantified. Confluent monolayers of Vero and hMSCs were infected with HSV-1 at 0.01 PFU per cell for 90 min at 37°C. Inoculums were harvested from both the cells at 10–40 h postinfection. The infectious virus-titer (PFU per milliliter) determined in triplicates in Vero cell by plaque assay indicates that the viral titer in cultured hMSCs increased overtime. Data represent the mean ± the standard deviation of results in triplicate wells in a representative experiment.
