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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 281312, 7 pages
http://dx.doi.org/10.1155/2011/281312
Research Article

Glucose Tolerance and Left Ventricular Pressure-Volume Relationships in Frequently Used Mouse Strains

Division of Experimental Cardiac Surgery, Department of Cardiovascular Diseases, K.U.Leuven, 3000 Leuven, Belgium

Received 14 September 2010; Revised 6 December 2010; Accepted 13 December 2010

Academic Editor: Oreste Gualillo

Copyright © 2011 Wouter Oosterlinck et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated glucose tolerance and left ventricular contractile performance in 4 frequently used mouse strains (Swiss, C57BL/6J, DBA2, and BalbC) at 24 weeks. Glucose tolerance was tested by measuring blood glucose levels in time after intraperitoneal glucose injection (2 mg/g body weight). Left ventricular contractility was assessed by pressure-conductance analysis. Peak glucose levels and glucose area under the curve were higher (all 𝑃 < . 0 5 ) in C57BL/6J ( 4 1 8 ± 6 5  mg/dL and 8 1 3 ± 1 0 0  mg·h/dL) versus Swiss ( 2 3 7 ± 6 6  mg/dL and 4 7 0 ± 1 2 6  mg·h/dL), DBA2 ( 1 1 3 ± 2 0  mg/dL and 3 0 4 ± 4 9  mg·h/dL, 𝑃 < . 0 1 ), and BalbC mice ( 1 7 4 ± 5 5  mg/dL and 4 1 6 ± 7 0  mg·h/dL). Cardiac output was higher (all 𝑃 < . 0 5 ) in Swiss ( 1 4 0 3 8 ± 4 5 3 0 μL/min) versus C57BL/6J ( 1 0 4 0 5 ± 2 6 8 3 μL/min), DBA2 ( 1 0 4 3 8 ± 3 2 5 1 μL/min), and BalbC mice ( 8 4 6 6 ± 3 0 1 3 μL/min). Load-independent left ventricular contractility assessed as recruitable stroke work (PRSW) was comparable in all strains. In conclusion, glucose tolerance and load-dependent left ventricular performance parameters were different between 4 mice background strains, but PRSW was comparable.