Research Article

Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells

Figure 2

Expression of pluripotency factors in SACK-D mPanstra cells. (a) Nuclear expression of Klf4 was evaluated by indirect in situ immunofluorescence (ISIF) with Klf4-specific antibodies. (b) Nuclear expression of Oct4 was evaluated by ISIF with Oct4-specific antibodies. In each analysis, mouse embryonic stem cells (mESCs) were analyzed as a positive control. DAPI, nuclear DNA fluorescence.
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(a)
312457.fig.002b
(b)