Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells
Figure 2
Expression of pluripotency factors in SACK-D mPanstra cells. (a) Nuclear expression of Klf4 was evaluated by indirect in situ immunofluorescence (ISIF) with Klf4-specific antibodies. (b) Nuclear expression of Oct4 was evaluated by ISIF with Oct4-specific antibodies. In each analysis, mouse embryonic stem cells (mESCs) were analyzed as a positive control. DAPI, nuclear DNA fluorescence.