Culture Environment-Induced Pluripotency of SACK-Expanded Tissue Stem Cells
Table 1
(a) Phenotypes of individual reprogrammed subclones from the mPanstra-25 cell strain following expansion in respective reprogramming conditions.
Condition
Phenotype
str, AP−
str, AP+
epl, AP−
epl, AP+
DFBS-Ctrl
23/23 (100%)
0/23 (0%)
0/23 (0%)
0/23 (0%)
0.06
0.00
0.00
0.00
DFBS-Xn
22/22 (100%)
0/22 (0%)
0/22 (0%)
0/22 (0%)
0.06
0.00
0.00
0.00
hiFBS-Ctrl
4/20 (20%)
1/20 (5%)
9/20 (45%)
6/20 (30%)
0.01
0.00
0.02
0.02
hiFBS-Xn
5/19 (26%)
1/19 (5%)
10/19 (53%)
3/19 (16%)
0.01
0.00
0.03
0.01
KOSR-Ctrl
0/16 (0%)
0/16 (0%)
14/16 (87.5%)
2/16 (12.5%)
0.00
0.00
0.04
0.01
KOSR-Xn
0/20 (0%)
0/20 (0%)
14/20 (70%)
6/20 (30%)
0.00
0.00
0.04
0.02
(b) Phenotypes of individual reprogrammed subclones from the mPanstra-12 cell strain following expansion in respective reprogramming conditions.
Condition
Phenotype
str, AP−
str, AP+
epl, AP−
epl, AP+
DFBS-Ctrl
8/21 (38%)
5/21 (24%)
8/21 (38%)
0/21 (0%)
0.05
0.03
0.05
0.00
DFBS-Xn
12/23 (52%)
4/23 (17%)
7/23 (31%)
0/23 (0%)
0.08
0.03
0.05
0.00
hiFBS-Ctrl
18/24 (75%)
0/24 (0%)
6/24 (25%)
0/24 (0%)
0.12
0.00
0.04
0.00
hiFBS-Xn
11/19 (58%)
0/19 (0%)
5/19 (26%)
3/19 (16%)
0.07
0.00
0.03
0.02
KOSR-Ctrl
4/14 (29%)
3/14 (21%)
1/14 (7%)
6/14 (43%)
0.03
0.02
0.01
0.04
KOSR-Xn
1/17 (6%)
1/17 (6%)
5/17 (29%)
10/17 (59%)
0.01
0.01
0.03
0.07
AP−: alkaline phosphatase negative; AP+: alkaline phosphatase positive; epl: epithelial-like; str: striated The number below each colony phenotype fraction corresponds to the estimated colony formation efficiency as a percentage of the number of cells used to initiate reprogramming cultures (see Figure 3(a)).
