Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization
Figure 3
Specificity of SP6 induction by MG132 treatment. (a) Subcellular localization of SP6 in C9 cells treated with MG132. SP6 localization was analysed by immunofluorescence signals. Red colour indicates SP6 detected by anti-HA antibody, blue colour shows nuclei stained with Hoechst 33342. (a–d): low magnification; (e–h): high magnification. Scale bar indicates 200 μm. (b) Knockdown of SP6 in MG132-treated C9 cells by siRNA. Posi: positive control; N: no treatment; M: Lipofectamine RNAiMAX only; GC: negative control siRNA; si: siRNA against Sp6. Upper panel: SP6 expression was detected by WB. Lower panel: relative SP6 levels estimated by densitometric analysis of WB results.
