Possible Linkage of SP6 Transcriptional Activity with Amelogenesis by Protein Stabilization
Figure 4
Screening of Sp6 target genes by microarray. (a) Venn diagram showing a summary of microarray data; the number of genes that was changed more than 2-fold between the SP6 producing sample (GC: negative control of siRNA) and the SP6 knockdown sample (siRNA of SP6) is also indicated. Numbers with brackets are unknown genes and those without brackets are known genes. The right panel indicates that the expression levels of tooth-related genes were normalized using a control (GC) based on microarray data. (b) Confirmation of microarray data by qPCR. The fold changes were calculated and compared to that of 24 h control (GC 24; 100%). GC: negative control siRNA; si: siRNA against Sp6. All experiments were performed in triplicate. (c) The mRNA levels of the Amtn and Rock1 genes in C9 at P7 and P28. qPCR was performed with total RNA purified from C9 cells at P7 and P28 and normalized by the level of 18s rRNA. The levels of Amtn and Rock1 mRNA expression at P7 are indicated as 100%.
