Using Bacterial Artificial Chromosomes in Leukemia Research: The Experience at the University Cytogenetics Laboratory in Brest, France
Figure 3
Identification of a new partner gene, FLNA, fused to MLL in acute myelomonoblastic leukemia. (a) FISH with dual-color MLL probe showing the insertion of chromosomal material within the disrupted signal of the MLL gene on the der(11). The yellow signal of the normal MLL is seen on the normal chromosome 11. (b) LDI-PCR of analyzed patient. The wild-type band in lane 2 does not appear. M: λ-Clal marker, 1: der(11), 2: der(reciprocal partner gene), *: derivative band. (c) FISH with BAC clones CTD-2238E23 (FLNA) (labeled in Spectrum Orange) and RP11-91A14 (MLL) (labeled in FITC) showing two green signals, two red signals and one yellow signal.
