Figure 2: Epo treatment and body weight gain and composition. C57BL/6 male mice (6 wks of age) were put on 60% high fat diet and received exogenous Epo treatment of the following doses (1000, 600, 300, 150, and 75 U/kg) injected subcutaneously, three times a week ( mice/group). At week 5, body weight gain (a) and body composition; lean mass (b) and fat mass (c) were determined for all groups and compared to lean and overweight mice injected with saline only as sham and normal diet controls, respectively. Results are expressed as the mean ± SEM. ANOVA single-factor test was used for statistical analysis; values of were considered statistically significant.