Figure 3: Epo-mediated improvement of glucose metabolism. C57BL/6 male mice (6 wks of age) were put on 60% high-fat diet and received exogenous Epo treatment of the following doses (1000, 600, 300, 150, and 75 U/kg) injected subcutaneously, three times a week; high-fat diet and control diet fed mice injected with saline only were used as sham and normal diet controls, respectively ( mice/group). Glucose tolerance test (a) was performed at week 5 after study onset; all mice were fasted for 16 hours and challenged with an intraperitoneal load of glucose (2 g/kg). Blood glucose was measured at 0, 15, 30, 60, 90, and 120 minutes after glucose administration. The effects of different Epo dose treatments on fasting blood glucose (b) and serum insulin (c) were also assessed. The results are expressed as the mean ± SEM. ANOVA single-factor test was used for statistical analysis; values of were considered statistically significant.