Figure 4: The effects of Epo treatment on food intake and physical activity. C57BL/6 male mice (6 wks of age) were put on 60% high-fat diet and received exogenous Epo treatment of the following doses (1000, 600, 300, 150, and 75 U/kg) injected subcutaneously, three times a week ( mice/group). After adapting to individual caging (i.e., one mouse per cage) for one week, the effects of Epo treatment on food intake (a) and physical activity (b) were determined for all groups and compared to lean and overweight mice injected with saline only as sham and normal diet controls, respectively. Results are expressed as the mean ± SEM. ANOVA single-factor test was used for statistical analysis; values of were considered statistically significant. Figure 4(b) inset summarizes the results of the linear regression analysis for total activity of all mice on high-fat diet receiving sham or Epo treatments (0, 150, 300, 600, and 1000 U/kg).