Research Article

Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem Cells

Figure 10

Cell culture of human embryonic stem cells as positive control. (a,b) Early cell clusters (magnification 100-times). (c) Developing cell cluster (magnification 100-times). (d) Developed cell cluster (magnification 100-times). (e,f) Cell clusters positively stained on alkaline phosphatase activity (magnification 100-times). (g) Cell cluster surrounded by small cells positively (brown) stained on the SOX-2 marker of pluripotency (magnification 100-times). (h) Cell cluster positively (brown) stained on surface antigen SSEA-4 characteristic for pluripotent stem cells (magnification 100-times). (Inverted microscope, Hoffman illumination.)
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