Ovarian Surface Epithelium in Patients with Severe Ovarian Infertility: A Potential Source of Cells Expressing Markers of Pluripotent/Multipotent Stem Cells
Figure 7
Alkaline phosphatase staining of cells and cell clusters during the culture of cells scraped from the ovarian surface epithelium. (a) Nonstained cell cluster (magnification 40-times). (b) Positively stained oocyte-like cell (arrow) (magnification 200-times). (c, d) Positively stained small round cells with diameters of 2 to 4 μm proliferating in a cell culture (magnification 200-times). (e, f) Positively stained cell clusters (magnification 40-times). (g) Positively stained cell cluster developing on the layer of autologous ovarian fibroblasts (magnification 100-times). (h) Small round cells (arrow) with diameters of 2 to 4 μm proliferating in the near surroundings (magnification 200-times). (inverted microscope, Hoffman illumination.)
