Dynamic Changes in Sarcoplasmic Reticulum Structure in Ventricular Myocytes
Figure 6
Expression of functional ryanodine receptors in motile corbular SR boutons. (a) Confocal image of a neonatal ventricular myocyte expressing tRFP-SR (left), labeled with BODIPY FL-X Ryanodine (center) and merged image (right). (b) Time-lapse confocal images of neonatal ventricular myocyte from panel A expressing tRFP-SR and labeled with BODIPY FL-X Ryanodine. Images shown were obtained 0, 150, and 300 seconds after the initiation of the experiment. Motile corbular SR boutons are labeled with colored circles. (c) Confocal image of adult ventricular myocyte expressing tRFP-SR (left), labeled with BODIPY FL-X Ryanodine (center) and merged image (right). (d) TIRF image (left) of a representative neonatal myocyte expressing tRFP-SR loaded with fluo-4. Green dot represents corbular SR bouton of interest. The plot in the center shows the time course of Ca2+ spark activity in this corbular SR bouton. The plot to the left shows the velocity of the corbular SR bouton marked by the green dot as a function of time. (e) Confocal image (left) of a representative adult ventricular myocyte expressing tRFP-SR loaded with fluo-4 AM. Colored dots mark corbular SR boutons in this cell. The two plots in this panel show the time-course of spontaneous Ca2+ sparks in corbular SR boutons i and ii.
