Dynamic Changes in Sarcoplasmic Reticulum Structure in Ventricular Myocytes
Figure 7
Microtubules regulate SR structure in neonatal and adult ventricular myocytes. (a) Confocal images of a neonatal ventricular myocyte expressing tRFP-SR in the absence (top; control) or presence of 10 μM nocodazole (bottom). (b) Confocal image of an adult ventricular myocyte expressing tRFP-SR. The inset shows a zoomed-in region of the cell delineated by the white box. The image to the left is a 2D Fourier transformation of the confocal image. (c) Histograms of SRPower values in control and nocodazole-treated adult ventricular myocytes. Dashed green line shows global shift in SRPower distributions in nocodazole-treated myocytes from the center (1.7 μm) of control myocytes. Solid red lines represent fits to the data with single (control; center = 1.7 μm) and dual (nocodazole; center peak 1 = 0.88 μm, center peak 2 = 1.53 μm) Gaussian functions.
