Combined Reversed Phase HPLC, Mass Spectrometry, and NMR Spectroscopy for a Fast Separation and Efficient Identification of Phosphatidylcholines

Table 3

Electrospray ionization efficiency of different fatty acid compared to undecanoic acid (𝑝𝐾_𝑠 = 4,69 for all acids).

Fatty acid	Number of carbons	Empirical formula	Ionisation efficiency* (%)
caprylic acid	8	C8H16O2	52
capric acid	10	C10H20O2	64
undecanoic acid	11	C11H22O2	100
lauric acid	12	C12H24O2	131
myristic acid	14	C14H28O2	194
palmitic acid	16	C16H32O2	285
stearic acid	18	C18H36O2	609
eicosanoic acid	20	C20H40O2	2300

^∗compared to undecanoic acid.