The Selection and Use of Sorghum (Sorghum propinquum) Bacterial Artificial Chromosomes as Cytogenetic FISH Probes for Maize (Zea mays L.)

Table 1

Sequenced maize CBM-RFLPs.

CBM	Marker/locus	Probe	Accession number	Type	Enzyme(s)	MaizeGDB insert; size (bp)	Sequenced RFLP; insert size
1.01	tub1	p-tub1	AY987961	Genomic	EcoRI/HindIII	156	156
1.02	umc157(chn)	p-umc157	DQ123890	Genomic	PstI	1220	1250
1.03	umc76(prob a)	p-umc76	AY751079	Genomic	PstI	760	710
1.04	asg45(ptk)	p-asg45	AY771210	Genomic	PstI	350	332
1.05	csu03	p-csu03	DQ123891	cDNA	EcoRI/XhoI	1200	1064
1.06	umc67a	p-umc67	AY771211	Genomic	PstI	650	644
1.07	asg62	p-asg62	DQ001865	Genomic	PstI	500	465
1.08	umc128	p-umc128	DQ123892	Genomic	PstI	740	755
1.09	cdj2	p-csu164	DQ642431	cDNA	EcoRI/XhoI	700	466
1.10	umc107a(croc)	p-umc107	DQ642432	Genomic	PstI	1090	1105
1.11	umc161a	p-umc161	AY771212	Genomic	PstI	700	723
1.12	bnl6.32^a	p-bnl6.32		Genomic	PstI	2250
2.01	bnl8.45a^b	p-bnl8.45		Genomic	PstI	2100
2.02	umc53a	p-umc53	AY771214	Genomic	PstI	640	604
2.03	umc06a	p-umc06	AY771213	Genomic	PstI	590	608
2.04	umc34	p-umc34	DQ001866	Genomic	PstI	970	934
2.05	umc131	p-umc131	AY771215	Genomic	PstI	810	859
2.06	umc255a	p-umc255	DQ123893	Genomic	PstI	1050	1013
2.07	umc005a	p-umc005	AY771216	Genomic	PstI	850	830
2.08	asg20	p-asg20	DQ123894	Genomic	PstI	550	337
2.09	umc049a	p-umc049	DQ123895	Genomic	PstI	630	627
2.10	php20581b(tb)	p-php20581	EU190456	Genomic	PstI	1400	1327
3.01	umc032a	p-umc32	DQ001867	Genomic	PstI	990	1019
3.02	csu32	p-csu32	DQ123896	Genomic	EcoRI/XhoI	500	411
3.03	asg24(gts)	p-asg24	AY771217	Genomic	PstI	550	372
3.04	asg48	p-asg48	DQ001868	Genomic	PstI	1600	1617
3.05	umc102	p-umc102	DQ005498	Genomic	PstI	1010	1023
3.06	bnl5.37a^b	p-bnl5.37		Genomic	PstI	2300
3.07	bnl6.16a^b	p-bnl6.16		Genomic	PstI	2450
3.08	umc17a	p-umc17	AY771218	Genomic	PstI	850	840
3.09	umc63a	p-umc63	DQ123897	Genomic	PstI	620	881
3.10	cyp1	p-csu25	DQ005499	cDNA	EcoRI/XhoI	1100	960
4.01	agrr115^c	p-agrr115		Genomic	EcoRI/HindIII	600
4.02	php20725a^b	p-php20725		Genomic	PstI	1650
4.03	umc31a	p-umc31	DQ123898	Genomic	PstI	550	582
4.04	npi386(eks)	p-npi386	DQ007988	Genomic	PstI	1200	1180
4.05	agrr37b^b	p-agrr37		cDNA	EcoRI/HindIII	949
4.06	umc156a	p-umc156	AY771219	Genomic	PstI	570	533
4.07	umc66a(lcr)	p-umc66	DQ007989	Genomic	PstI	1020	1036
4.08	umc127c	p-umc127	DQ642433	Genomic	PstI	1210	649
4.09	umc52	p-umc52	DQ123899	Genomic	PstI	1500	824
4.10	php20608a	p-php20608	DQ007990	Genomic	PstI	780	1451
4.11	umc169	p-umc169	DQ123900	Genomic	PstI	670	813
5.01	npi409^b	p-npi409		Genomic	PstI	710
5.02	umc90	p-umc90	DQ642434	Genomic	PstI	1240	1226
5.03	tub4	p-tub4	DQ007991	cDNA	EcoRI/HindIII	200	230
5.04	bnl4.36	p-bnl4.36	DQ642435	Genomic	PstI	2300	2210
5.05	csu93b	p-csu93	DQ015673	cDNA	EcoRI/XhoI	800	677
5.06	umc126a	p-umc126	AY771220	Genomic	PstI	670	663
5.07	umc108	p-umc108	DQ642436	Genomic	PstI	970	958
5.08	bnl5.24a^a	p-bnl5.24		Genomic	PstI	2500
5.09	php10017	p-php10017	AY771221	Genomic	PstI	470	526
6.01	umc85a	p-umc85	AY772450	Genomic	PstI	600	561
6.02	umc59a^a	p-umc59		Genomic	PstI	930
6.03	npi393	p-G23A-06	DQ015674	Genomic	PstI	1200	1249
6.04	umc65a	p-umc65	AY772451	Genomic	PstI	650	691
6.05	umc21	p-umc21	DQ123901	Genomic	PstI	1050	1062
6.06	umc38a	p-umc38	DQ059316	Genomic	PstI	1010	1022
6.07	umc132a(chk)	p-umc132	DQ1238902	Genomic	PstI	500	472
6.08	asg7a	p-asg7	DQ642437	Genomic	PstI	550	353
7.01	asg8(myb)	p-asg8	DQ642438	Genomic	PstI	500	320
7.02	asg34a(msd)^a	p-asg34		Genomic	PstI	1350
7.03	asg49	p-asg49	DQ059317	Genomic	PstI	400	400
7.04	umc254	p-umc254	EU190457	Genomic	PstI	1050	1000
7.05	umc245	p-umc245	AY772452	Genomic	PstI	700	665
7.06	umc168	p-umc168	DQ059318	Genomic	PstI	1080	1072
8.01	npi220a	p-G10F-01	DQ059319	Genomic	HindIII	400	406
8.02	bnl9.11a(lts)^a	p-bnl9.11		Genomic	PstI	2400
8.03	umc124a(chk)	p-umc124	DQ059320	Genomic	PstI	1160	1162
8.04	bnl7.08a^a	p-bnl7.08		Genomic	PstI	2300
8.05	bnl2.369^a	p-bnl2.369		cDNA	EcoRI	700
8.06	csu31a^a	p-csu31		cDNA	EcoRI/XhoI	800
8.07	npi268a	p-npi268	DQ123903	Genomic	PstI	710	688
8.08	npi414a	p-npi414	AY772453	Genomic	PstI	870	893
8.09	agrr21^a	p-agrr21		cDNA	EcoRI/HindIII	899
9.01	umc109	p-umc109	AY772454	Genomic	PstI	840	797
9.02	bz1^a	p-umc192		cDNA	PstI	1750
9.03	wx1^a	p-umc25		cDNA	EcoRI	2300
9.04	csu147^a	p-csu147		cDNA	EcoRI/XhoI	800
9.05	umc95	p-umc95	AY772455	Genomic	PstI	680	660
9.06	csu61a	p-csu61		cDNA	EcoRI/XhoI	500
9.07	asg12	p-asg12	DQ123904	Genomic	PstI	700	647
9.08	csu54b^a	p-csu54		cDNA	EcoRI/XhoI	1400
10.01	php20075a(gast)	p-php20075	DQ059321	Genomic	PstI	1400	1311
10.02	npi285a(cac)^a	p-npi285		Genomic	PstI	1250
10.03	umc130	p-umc130	DQ059322	Genomic	PstI	640	634
10.04	umc64a	p-umc64		Genomic	PstI	710
10.05	umc259a	p-umc259	DQ123905	Genomic	PstI	550	579
10.06	umc44a	p-umc44	AY772456	Genomic	PstI	800	794
10.07	bnl7.49a(hmd)^a	p-bnl7.49		Genomic	PstI	2100

aFull length RFLP sequence not available.
bUnable to sequence with vector primers.
cPoly A tail at both ends of insert sequence.