414198.fig.003a
(a)
414198.fig.003b
(b)
Figure 3: (a) Western blot analysis showing the expressed NS1 recombinant protein of ~28 kDa in expressed GS115 Pichia pastoris strain using NS1 polyclonal antibody as a primary antibody; expression started at day 7 after methanol induction; (b) SDS-PAGE separating the proteins into different molecular weight prior to transfer to NC membrane and immunodetection.