Cloning and Expression of Highly Pathogenic Avian Influenza Virus Full-Length Nonstructural Gene in Pichia pastoris
Figure 3
(a) Western blot analysis showing the expressed NS1 recombinant protein of ~28 kDa in expressed GS115 Pichia pastoris strain using NS1 polyclonal antibody as a primary antibody; expression started at day 7 after methanol induction; (b) SDS-PAGE separating the proteins into different molecular weight prior to transfer to NC membrane and immunodetection.
