419343.fig.001a
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419343.fig.001b
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419343.fig.001c
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419343.fig.001d
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419343.fig.001e
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419343.fig.001f
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419343.fig.001g
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419343.fig.001h
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Figure 1: UA inhibited proliferation and induced apoptosis in gastric cancer cell BGC-803. (a) Inhibition of proliferation of BGC-803 was assessed by MMT staining. (b) Morphological changes of BGC-803 cells after UA (IC50 (24 h)) treatment for 24 h (Bar represents 200 μm); magnified image of cells was showed in corner (Bar represents 200 μm). (c) H&E staining of BGC-803 cells after UA (IC50 (24 h)) treatment for 24 h (Bar represents 200 μm), and arrows indicated apoptotic cells; magnified image of cells was showed in corner (Bar represents 200 μm). (d) DNA ladder was found in BGC-803 cells treated with UA (IC50 (24 h) and IC50 (36 h)), not in BGC-803 cells treated with 0.2% DMSO or without treatment. (e) Apoptosis of BGC-803 cells induced by UA was detected by Annexin-V staining and flow cytometry. (f) was the quantified data from experiment showed in (e). Apoptotic rate was , , and in control, DMSO, and UA treated cells, respectively, (* ). (g) Western blot showed expression of apoptotic related genes in BGC-803 cell treated with DMSO, UA and without treatment; β-actin was used as internal control. (h) showed related amount of protein from (g) (* ).