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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 421701, 8 pages
http://dx.doi.org/10.1155/2011/421701
Methodology Report

A High-Throughput Solid-Phase Microplate Protein-Binding Assay to Investigate Interactions between Myofilament Proteins

1Department of Physiology and Cell Biology, The Ohio State University, Columbus, OH 43210, USA
2Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA

Received 25 May 2011; Accepted 24 August 2011

Academic Editor: Xupei Huang

Copyright © 2011 Brandon J. Biesiadecki and J.-P. Jin. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. B. J. Biesiadecki, B. D. Elder, Z. B. Yu, and J. P. Jin, “Cardiac troponin T variants produced by aberrant splicing of multiple exons in animals with high instances of dilated cardiomyopathy,” Journal of Biological Chemistry, vol. 277, no. 52, pp. 50275–50285, 2002.
  2. B. J. Biesiadecki and J. P. Jin, “Exon skipping in cardiac troponin T of turkeys with inherited dilated cardiomyopathy,” Journal of Biological Chemistry, vol. 277, no. 21, pp. 18459–18468, 2002. View at Publisher · View at Google Scholar · View at Scopus
  3. J. P. Jin and S. M. Chong, “Localization of the two tropomyosin-binding sites of troponin T,” Archives of Biochemistry and Biophysics, vol. 500, no. 2, pp. 144–150, 2010. View at Publisher · View at Google Scholar · View at Scopus
  4. B. J. Biesiadecki, K. L. Schneider, Z. B. Yu, S. M. Chong, and J. P. Jin, “An R111C polymorphism in wild turkey cardiac troponin I accompanying the dilated cardiomyopathy-related abnormal splicing variant of cardiac troponin T with potentially compensatory effects,” Journal of Biological Chemistry, vol. 279, no. 14, pp. 13825–13832, 2004. View at Publisher · View at Google Scholar · View at Scopus
  5. B. You, G. Yan, Z. Zhang et al., “Phosphorylation of cardiac troponin I by mammalian sterile 20-like kinase 1,” Biochemical Journal, vol. 418, no. 1, pp. 93–101, 2009. View at Publisher · View at Google Scholar · View at Scopus
  6. Z. Zhang, B. J. Biesiadecki, and J. P. Jin, “Selective deletion of the NH2-terminal variable region of cardiac troponin T in ischemia reperfusion by myofibril-associated μ-calpain cleavage,” Biochemistry, vol. 45, no. 38, pp. 11681–11694, 2006. View at Publisher · View at Google Scholar · View at Scopus
  7. P. L. Engel, T. Kobayashi, B. Biesiadecki et al., “Identification of a region of troponin I important in signaling cross-bridge-dependent activation of cardiac myofilaments,” Journal of Biological Chemistry, vol. 282, no. 1, pp. 183–193, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. J. P. Jin, S. M. Chong, and M. M. Hossain, “Microtiter plate monoclonal antibody epitope analysis of Ca2+- and Mg2+-induced conformational changes in troponin C,” Archives of Biochemistry and Biophysics, vol. 466, no. 1, pp. 1–7, 2007. View at Publisher · View at Google Scholar · View at Scopus
  9. J. P. Jin and K. Wang, “Nebulin as a giant actin-binding template protein in skeletal muscle sarcomere. Interaction of actin and cloned human nebulin fragments,” FEBS Letters, vol. 281, no. 1-2, pp. 93–96, 1991. View at Publisher · View at Google Scholar · View at Scopus
  10. O. Ogut and J. P. Jin, “Developmentally regulated, alternative RNA splicing-generated pectoral muscle-specific troponin T isoforms and role of the NH2-terminal hypervariable region in the tolerance to acidosis,” Journal of Biological Chemistry, vol. 273, no. 43, pp. 27858–27866, 1998. View at Publisher · View at Google Scholar · View at Scopus
  11. O. Ogut and J. P. Jin, “Expression, zinc-affinity purification, and characterization of a novel metal-binding cluster in troponin T metal-stabilized alpha-helical structure and effects of the NH2-terminal variable region on the conformation of intact troponin T and its association with tropomyosin,” Biochemistry, vol. 35, no. 51, pp. 16581–16590, 1996. View at Scopus
  12. J. P. Jin, M. P. Walsh, C. Sutherl, and W. Chen, “A role for serine-175 in modulating the molecular conformation of calponin,” Biochemical Journal, vol. 350, no. 2, pp. 579–588, 2000. View at Publisher · View at Google Scholar · View at Scopus
  13. J. P. Jin, D. Wu, J. Gao, R. Nigam, and S. Kwong, “Expression and purification of the h1 and h2 isoforms of calponin,” Protein Expression and Purification, vol. 31, no. 2, pp. 231–239, 2003. View at Publisher · View at Google Scholar · View at Scopus
  14. J. P. Jin, “Cloned rat cardiac titin class I and class II motifs. Expression, purification, characterization, and interaction with F-actin,” Journal of Biological Chemistry, vol. 270, no. 12, pp. 6908–6916, 1995. View at Scopus
  15. J. Wang and J. P. Jin, “Conformational modulation of troponin T by configuration of the NH2- terminal variable region and functional effects,” Biochemistry, vol. 37, no. 41, pp. 14519–14528, 1998. View at Publisher · View at Google Scholar · View at Scopus
  16. K. Gregorius, S. Mouritsen, and H. I. Elsner, “Hydrocoating: a new method for coupling biomolecules to solid phases,” Journal of Immunological Methods, vol. 181, no. 1, pp. 65–73, 1995. View at Publisher · View at Google Scholar · View at Scopus
  17. Z. Zhang, S. Akhter, S. Mottl, and J. P. Jin, “Calcium-regulated conformational change in the C-terminal end segment of troponin I and its binding to tropomyosin,” FEBS Journal, vol. 278, no. 18, pp. 3348–3359, 2011.
  18. O. Ogut and J. P. Jin, “Cooperative interaction between developmentally regulated troponin T and tropomyosin isoforms in the absence of F-actin,” Journal of Biological Chemistry, vol. 275, no. 34, pp. 26089–26095, 2000. View at Publisher · View at Google Scholar · View at Scopus
  19. S. Akhter, Z. Zhang, and J. P. Jin, “The NH2-terminal extension regulates molecular conformation and function of cardiac troponin I,” Biophysical Journal, vol. 100, no. 3, supplement, pp. 370a–371a, 2011.