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424929.fig.005b
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Figure 5: Light and immunofluorescence microphotographs of thrombus on rabbit femoral arteries. Representative light and immunofluorescence microphotographs of thrombi that developed in normal femoral artery 15 minutes after balloon injury and in atherosclerotic neointima from rabbits on 0.5% cholesterol diet. Whole images of arterial sections of normal artery (a) or atherosclerotic artery (b) 15 minutes after balloon injury. The size of thrombus significantly differs between normal artery thrombus and atherothrombus (*). The normal artery thrombus is too small to recognize. Hematoxylin eosin stain. Rows (c, d) show differential interference contrast, fluorescein isothiocyanate-labeled GPIIb/IIIa (green), Cy3-labeled fibrin (red), and merged immunofluorescent images. Areas with colocalized factors are stained yellow. Thrombi on normal intima are composed of small aggregated platelets (c), whereas those on atherosclerotic neointima comprise platelets and fibrin (d). I, intima; M, media; IEL, internal elastic lamina (from Yamashita et al. [28] with permission).