435271.fig.007
Figure 7: HMM density (ρ) dependence of maximum unloaded filament sliding speed ( ) of regulated thin filaments reconstituted with HcTn and either α-GS-Tm WT (○), α-GS-Tm D175N (▲), or α-GS-Tm E180G (■). was obtained at each ρ according to the method of Uyeda et al. [61] as described in Section 2.8; each point is from one flow cell. Note that for thin filaments containing α-GS-Tm D175N is shifted leftward of that for thin filaments containing either α-GS-Tm WT or α-GS-Tm E180G.