Facilitated Cross-Bridge Interactions with Thin Filaments by Familial Hypertrophic Cardiomyopathy Mutations in α-Tropomyosin

Table 1

Motility speed-pCa regression parameter estimates for regulated thin filaments containing HcTn and WT or mutant tropomyosin.

	α-GS-Tm	α-GS-Tm	α-GS-Tm	α-GS-Tm
	WT	V95A	D175N	E180G
pCa50	6.52±0.07	6.63±0.03 *	6.72±0.12	7.16±0.03 *
𝑁	2.3±0.7	2.5±0.4	1.4±0.5 *	1.7±0.2*

Speed-pCa data from regulated motility assays (Figure 3) were fit to (4) by nonlinear least squares regression (Methods). Values are regression parameter estimates ±SE. Parameter estimates of pCa₅₀ and 𝑛 that are significantly different from those for α-GS-Tm WT are indicated by *(𝑃<0.05).