Table 1: Motility speed-pCa regression parameter estimates for regulated thin filaments containing HcTn and WT or mutant tropomyosin.

α-GS-Tmα-GS-Tmα-GS-Tmα-GS-Tm
WTV95AD175NE180G

pCa50 * *

* 1 *

Speed-pCa data from regulated motility assays (Figure 3) were fit to (4) by nonlinear least squares regression (Methods). Values are regression parameter estimates ±SE. Parameter estimates of pCa50 and that are significantly different from those for α-GS-Tm WT are indicated by *( ).