Table 2: Estimates of structural content of WT and mutant tropomyosins.
α-helix (%)β-sheet (%)β-turn (%)Unordered (%)
[θ]222CDProCDProCDProCDPro
α-GS-Tm WT 8 4 . 5 ± 4 . 6 7 9 . 1 ± 1 0 . 8 4 . 3 ± 3 . 4 7 . 0 ± 6 . 7 1 0 . 4 ± 5 . 8
α-GS-Tm V95A 6 0 . 6 ± 1 0 . 4 6 2 . 7 ± 6 . 4 8 . 2 ± 3 . 2 1 2 . 2 ± 3 . 5 1 7 . 0 ± 5 . 1
α-GS-Tm D175N 7 2 . 1 ± 0 . 7 7 2 . 9 ± 7 . 0 5 . 6 ± 3 . 7 1 1 . 3 ± 5 . 5 1 3 . 3 ± 6 . 0
α-GS-Tm E180G 6 9 . 7 ± 3 . 3 6 7 . 9 ± 5 . 6 5 . 9 ± 2 . 7 9 . 5 ± 4 . 7 1 7 . 3 ± 3 . 7
β-GS-Tm WT 8 3 . 6 ± 6 . 4 7 7 . 8 ± 1 3 . 1 5 . 2 ± 4 . 4 7 . 8 ± 6 . 5 1 0 . 1 ± 8 . 8
Estimates of structural content for each of the five recombinant Tm’s were obtained from analyses of the individual CD spectra that were averaged for Figure 4. α-helical content was estimated from [θ]222 (Methods). Content of α-helix, β-sheet, and unordered structure was obtained using CDPro software (Methods). Values are mean ± S.D. ( 𝑁 = 2 - 3 spectra); note that, for CDPro estimates, S.D. is the mean of S.D. values from CDPro.