WF10 Stimulates NK Cell Cytotoxicity by Increasing LFA-1-Mediated Adhesion to Tumor Cells
Figure 2
WF10 specifically affects NK cells. (a) Freshly isolated resting human NK cells were preincubated for 5 h with or without 200 M WF10 in culture medium, washed and then used in a standard 4 h 51Cr-release assays against LCL721.221 target cells at the indicated effector to target (E/T) ratios. (b) LCL721.221 target cells were preincubated with or without 200 M WF10 in culture medium, washed and then used as targets in a standard 4 h 51Cr-release assays using freshly isolated human NK cells as effectors. The activity of the WF10-treated cells is shown relative to the cytotoxic activity of control cells (set as 100%) as an average value from (a) 4 or (b) 3 independent experiments.
