WF10 Stimulates NK Cell Cytotoxicity by Increasing LFA-1-Mediated Adhesion to Tumor Cells
Figure 6
WF10 enhances NK cell adhesion. (a) NK cells and 721.221 cells were labeled with a different fluorescent dye, washed, and then incubated together with or without WF10 (200 or 600 M). At the indicated time points the mixed suspension was fixed with ice-cold 4% PFA and analyzed by FACS. The amount of NK: 721.221 conjugates formed by the cells without WF10 was used as a reference (100%). Average values and the standard deviation from 3 independent experiments with different blood donors are shown. (b, c) Freshly isolated NK cells were incubated with PE-conjugated ICAM-1 complex (scICAM-1) with or without WF10 for 3 h. Samples were either left unstimulated (b, left panel), or treated with IL-15 (100 U/ml) (b, right panel). The amount of bound ICAM-1 complexes was measured by FACS. A representative experiment is shown in (b). The ICAM-1 binding to control cells (MFI ) and IL-15 stimulated cells (MFI ) was used as a reference (100%). (c) Average values and standard deviations from 5 independent experiments with different blood donors are shown. The ratio of each outcome and its control was tested against a value of 1 (equality) by two-sided single-sample -tests (* ).
