Construction and Characterization of a Bacterial Artificial Chromosome Library for the A-Genome of Cotton (G. arboreum L.)

Figure 3

The scheme representations of BAC pooling and PCR-based screening strategy. “→” indicated the direction of BAC pooling. Step I, one column pool was mixed with each 384-well column and 16 clones in the same column (gray dot) were combined into a column pool. Step II, One super pool was mixed with each column pool in the same raw and the column pools in same raw (black dot) were mixed. The entire BAC library of 123, 648 clones on 322 384-well plates was organized into 322 super pools. “⇢” indicated the direction of three rounds of PCR-based screening. Round I, screening against 322 Super pools. Round II, screening against the individual row of column pools. Round III, screening against the individual column of particular plate(s).