- About this Journal ·
- Abstracting and Indexing ·
- Advance Access ·
- Aims and Scope ·
- Annual Issues ·
- Article Processing Charges ·
- Articles in Press ·
- Author Guidelines ·
- Bibliographic Information ·
- Citations to this Journal ·
- Contact Information ·
- Editorial Board ·
- Editorial Workflow ·
- Free eTOC Alerts ·
- Publication Ethics ·
- Reviewers Acknowledgment ·
- Submit a Manuscript ·
- Subscription Information ·
- Table of Contents
Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 464916, 15 pages
One Function—Multiple Mechanisms: The Manifold Activities of p53 as a Transcriptional Repressor
1Molecular Oncology, Department of Obstetrics and Gynecology, University of Leipzig, Semmelweisstr. 14, 04103 Leipzig, Germany
2Medical Biochemistry and Molecular Biology, University of Saarland, Medical Center, Building 44, 66421 Homburg, Germany
Received 21 September 2010; Accepted 17 January 2011
Academic Editor: Noel F. Lowndes
Copyright © 2011 Levin Böhlig and Karen Rother. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Maintenance of genome integrity is a dynamic process involving complex regulation systems. Defects in one or more of these pathways could result in cancer. The most important tumor-suppressor is the transcription factor p53, and its functional inactivation is frequently observed in many tumor types. The tumor suppressive function of p53 is mainly attributed to its ability to regulate numerous target genes at the transcriptional level. While the mechanism of transcriptional induction by p53 is well characterized, p53-dependent repression is not understood in detail. Here, we review the manifold mechanisms of p53 as a transcriptional repressor. We classify two different categories of repressed genes based on the underlying mechanism, and novel mechanisms which involve regulation through noncoding RNAs are discussed. The complete elucidation of p53 functions is important for our understanding of its tumor-suppressor activity and, therefore, represents the key for the development of novel therapeutic approaches.
Complex regulatory systems govern the process of genomic integrity maintenance. Intracellular and extracellular stress signals can lead to induction of central signalling proteins which generate different downstream effects. The transcription factor p53 is the most important known tumor suppressor, and functional inactivation of p53 is frequently associated with loss of genomic stability . Originally thought to be an oncogene due to its binding to the SV40 T-antigen [2, 3], p53 later turned out to be a very potent apoptosis inducer and inhibitor of cell-cycle progression . p53 knockout mice are susceptible to different tumor types  and restoration of p53-function leads to the regression of tumors in vivo [6, 7]. The tumor suppressive function of p53 is mainly attributed to its ability to regulate numerous target genes at the transcriptional level. Functions both as a transactivator and as a repressor have been described for p53.
The mechanism of transcriptional induction of p53 is well characterized. It involves DNA-binding to the p53 consensus site . Transactivation is achieved by interactions with components of the general transcription factor TFIID like the TATA box binding protein (TBP) [9, 10] and TAFII31 . Numerous target genes upregulated by p53 have been characterized so far. Well-known targets are involved in apoptosis like Bax  and in cell-cycle control like the cyclin dependent kinase (CDK) inhibitor p21CIP1  or the inhibitor of Cyclin B/Cdc2 nuclear import, 14-3-3σ . For all known upregulated target genes DNA-binding of p53 is essential for regulation of gene transcription.
In addition to transcriptional induction, p53 has been shown to repress various target genes, but obviously by different mechanisms . Many of them are involved in cell-cycle control (Figure 1) and contribute to p53-induced cell-cycle arrest. Gene expression analyses showed that p53-dependent repression of target genes is associated with apoptosis after hypoxia treatment, whereas transactivated genes were clearly underrepresented . Therefore, gene repression by p53 contributes to its tumor suppressive activity. In this paper we will summarize the wide diversity of molecular mechanisms of p53-dependent repression described to date. In principle, two categories of p53-repressed target genes can be classified: (I) genes that are regulated by direct interaction of p53 with target gene promoters or bound cofactors, (II) genes that are indirectly regulated by other p53 target genes. But most (if not all) of the mechanisms of transcriptional repression by p53 require its intact DNA-binding domain. Whether the DNA-binding domain is always needed for direct DNA binding to target gene promoters or interaction of p53 with other proteins through this domain can evoke p53-dependent repression, remains to be elucidated.
2. Diversity of p53-Binding Sites
The classical consensus site of a p53 binding element consists of two repeats of the palindromic sequence RRRCWWGYYY (in which R is a purine, Y is a pyrimidine and W is an adenine or thymine) which are separated by a spacer of 0–13 base pairs. p53-binding sites which match this consensus sequence are extensively described in p53-dependent transactivation. Hundreds of p53 target genes (encoding both proteins and noncoding RNAs) have been discovered up to date [17–19] and this number still increases. Among the endogenous human p53 response elements that have been validated by p53 binding and corresponding gene expression, nearly 95% have mismatches from the consensus sequence . However, these mismatches were not considered significant to result in a change of the transcriptional activation function of p53 .
Analogous to transcriptional activation, p53 may repress target genes by binding to a consensus element in the promoter region. In most cases they differ from the canonical consensus sequence. Recently, a specific variation in the central dinucleotide core within the p53-response element was identified to determine whether p53 acts as a transcriptional activator or as a repressor on the target gene . The p53 binding site mediates strong activation of the respective promoters when the central dinucleotide is AT, AA or TT, matching the classical consensus sequence. Core dinucleotide combinations of TG, CA, CC, CG, GG or GC are found in repressive p53 response elements . The function of the remaining dinucleotides depends on the flanking nucleotides of the response element and can either mediate p53-dependent activation or repression whereas the position nearest to the CWWG core motif has the strongest positional effect . In addition to variations within the two decamers of the p53 binding site, the spacer between both half sites also influence the p53 effect. Recent analyses of validated repressor sites demonstrate often longer spacers than activator sites .
In addition new p53 binding elements have been found allowing direct interaction of p53 with promoter DNA (Figure 2, panel I). One example is transcriptional repression of the MDR promoter , where p53 can repress transcription directly by binding to a novel head-to-tail (HT) site within the MDR1 promoter. A similar element was found in the CD44 promoter which is efficiently repressed by p53 in a DNA-binding-dependent manner . Work from Marks et al. showed that the rat Bradykinin B2 promoter harbours two binding sites and one of them was responsible for p53-dependent induction while the other mediated transcriptional repression by p53. Intriguingly, the function of the repressive element was strongly dependent upon the promoter context as it turned into an activating element when cloned in front of a TATA-Luc minimal promoter . This mechanism of p53-dependent repression therefore may involve other factors, recruited to the target gene promoters dependent on sequence binding motifs or epigenetic modifications of chromatin.
3. Repression by Direct Interactions with Target Gene Promoters or Bound Cofactors
3.1. DNA-Binding-Dependent Mechanisms
Different mechanisms which are dependent on a direct DNA binding of p53 have been described (Figure 2). One possibility involves binding of p53 to an element which overlaps binding sites from coactivator molecules (Figure 2, panel II). But direct DNA-binding of p53 to such an element and subsequent interference with the activity of other transcription factors is rarely the exclusive mechanism for p53-dependent repression. Instead, in most cases manifold distinct molecular mechanisms contribute concertedly to p53-dependent response of its repressed target genes. However, in this section we will mention those p53 target genes, whose transcriptional repression is achieved at least in part by direct binding of p53 to their respective promoters. Additional mechanisms described for these p53 target gene will be discussed in detail in later sections.
The expression of the alpha-fetoprotein (AFP) has been shown to be governed by hepatic nuclear factor 3 (HNF3) which binds to a sequence called the developmental repression domain of the promoter. Due to its higher binding affinity, p53 is able to displace HNF3 from the AFP promoter. Furthermore, it was demonstrated that p53 can interfere actively with transcription of AFP independently of HNF3 most likely through recruitment of a corepressor complex . However, this mechanism occurred exclusively in liver cells . Wilkinson et al. proved that SnoN is the corepressor whose interaction with histone deacetylases (HDACs) is promoted by p53 . Furthermore, they demonstrated additional crosstalk between the TGF-β and p53 signalling pathways. Both, p53- and TGF-β-activated SMAD transcription factors are able to bind to an intercalating response element in the AFP promoter. The interaction of this complex with SnoN and the corepressor mSin3A is essential for repression of AFP transcription . mSin3A is a mediator of HDAC recruitment which induce changes in chromatin structure. Therefore, the protein complex needed for the repression of the AFP promoter contains several proteins which act on the local chromatin structure. These results indicate that different mechanisms govern repression of the AFP promoter by p53 and that tissue-specificity is achieved through recruitment of specific corepressor proteins.
Recently a further p53 target was identified to be regulated by p53 through a similar mechanism. Activity of the human polycystic kidney disease-1 (PKD-1) promoter was shown to be repressed by direct binding of p53 to four p53-response elements (BS1-4) . One of these sites (BS1) dictates active repression in association with an HDAC/mSin3A repressor complex. However, BS1 requires all three other p53-binding sites for full activity, and the authors suggest a model whereby p53 exerts a biphasic control on PKD1 gene transcription, depending on cellular context and the cognate cis-acting element.
A mechanism involving direct binding of p53 to a promoter element which overlaps a binding site from a coactivator molecule was also shown for the human AP-endonuclease (APE1/Ref-1) promoter. APE1 is an essential multifunctional protein, and plays a central role in the repair of oxidative base damage via the DNA base excision repair (BER) pathway. A recent study demonstrated that endogenous p53 is bound to the APE1 promoter region that includes a SP1 site, and that this binding of p53 interferes with SP1 binding to the APE1 promoter .
Such an interference of direct p53 DNA-binding with function of general transcription factor SP1 is described to contribute to p53-dependent repression of several other p53 target genes, including Cdc25C and Cyclin B1. Repression of both genes were found to be carried out at least in part by binding of p53 in vicinity to an adjacent SP1-binding site and interaction of these two factors (Figure 2, panel III) [29, 30]. However, several further mechanisms have been described to contribute to p53-dependent repression of both genes. For Cdc25C, intact CDE/CHR elements (cell-cycle-dependent element/cell-cycle genes homology region)  or CCAAT boxes of the promoter [31–33] are found to be required and/or recruitment of histone- and promoter-modifying enzymes is demonstrated [34, 35]. In addition, for Cdc25C indirect mechanism of p53-dependent repression via the target gene p21CIP1 was described . These different types of mechanisms are discussed below.
p53 activity has also been shown to interfere with the transcription of viruses. The HIV-LTR region was shown to be bound by p53 with partial displacement of SP1 leading to transcriptional repression . Another example for overlapping transcription factor binding sites is the survivin gene . The mechanism of p53-dependent repression involves direct binding of p53 to the promoter to a sequence which includes an E2F binding site. Hoffman et al. showed that increasing levels of p53 are able to overcome E2F-induced survivin expression, suggesting a displacement of E2F from the promoter . Later experimental work by Löhr et al. found that direct binding of p53 is not essential for repression . Instead, an indirect mechanism was proposed involving the p53 target gene p21CIP1 which is discussed in detail below. Raj and coworkers showed that the regulation of survivin in melanocytes is carried out by different mechanisms also under nonstress conditions . One of these involves direct p53-binding to the promoter and disruption of this interaction is sufficient to induce basal survivin expression. While it has been reported that p53 also affects survivin expression via the p21CIP1-Rb-E2F pathway mediated by E2F1 and E2F3 [36, 38, 40], Raj and coworkers also demonstrated binding of E2F2 to a newly identified E2F-binding site within the survivin promoter . This implicates a novel role for E2F2 rather than E2F1 or E2F3  in the negative regulation of survivin expression in human melanocytes. Moreover, in this cell system, p53-dependent repression of the survivin promoter appeared to be independent of the retinoblastoma protein Rb . Furthermore, a recent study demonstrated that hypermethylation of the survivin promoter in endometrial tumors is correlated with increased survivin expression. The authors speculated that DNA-methylation could inhibit the direct binding of p53 to the survivin promoter. In addition microarray data were presented, which suggested that de-repression by methylation is a general mechanism of p53 regulation . In conclusion, there seem to be different mechanisms governing p53-dependent regulation of survivin expression which might be dependent on the cell type employed for the studies as well as on stress conditions affecting the cells.
In addition to these primary p53 target genes, for a couple of genes direct DNA-binding of p53 to the promoter region in vivo was demonstrated for example for the p202- , PTTG1- , PRC1- , CHEK- , RAD51- [46, 47], hDDA3- , Hsp90β- , and LASP1-gene . Here interaction of p53 with its consensus sequence leads to transcriptional repression. However, the underlying mechanisms were not investigated further.
3.2. DNA-Binding-Independent Mechanisms
Beside the mechanism relying on direct DNA-binding numerous target genes have been described where p53 regulates promoter activity but direct DNA-binding is not required. We classified these mechanisms roughly into two groups: one comprises interaction with other transcription cofactors (Figure 3, panel I) or interaction with the basal transcription machinery (Figure 3, Panel II), the second covers regulation of chromatin structure and promoter methylation (Figure 4).
Different evidences show that p53 is able to bind to a variety of coactivators and interferes with their transactivator function. Promoter regulation of the hTERT- , insulin receptor- , IGF-I receptor- , VEGF-  and hGR1-genes  depends on binding of p53 to SP1. This protein-protein interaction impairs binding of SP1 to promoter DNA leading to decreased promoter activity. Binding of p53 to the promoter was not detected for these target genes.
It has been known for some time, that matrix metalloproteases are repressed by p53  and mechanistic investigations had been carried out showing that p53 interacts with promoter-bound AP-1. Although p53 did not modulate AP-1 binding to its consensus sequence, the repressive effect was completely abrogated after deletion of the AP-1 binding site . Thus, this repression mechanism seems to depend rather on the modulation of AP-1 activity than on competitive promoter-binding.
The CCAAT-Box binding factor NF-Y has been implicated in the repression of several cell-cycle genes. In addition to the Cyclin B1 gene mentioned before, repression of its homologue, Cyclin B2 , has been attributed to NF-Y function, too . p53 binds to the NF-YC subunit of NF-Y which is recruited to cell-cycle promoters of the Cyclin B2, Cdc25C and Cdc2 genes. Following DNA-damage, p53 is acetylated and induces changes in chromatin structure through recruitment of histone deacetylases HDAC1, 4 and 5 [31, 34]. The NF-Y binding sites are essential for both, p53-dependent repression and HDAC-recruitment. These results indicate that repression of at least some cell-cycle promoters is dependent on intact binding of NF-Y to CCAAT-Boxes and in this context NF-Y bound p53 is part of a large chromatin remodelling repressor complex. Other experiments showed, however, reduced basal activity after mutation of the CCAAT-Boxes of the Cyclin B2 promoter. Repression by p53, however, was only moderately impaired , which implies that there are different mechanisms leading to Cyclin B2 repression. Another group showed that NF-Y binding to the Cyclin B1 and Cdc2 promoters itself is impaired after overexpression of p53 . In addition, the transcriptional repression of the Cdc2-gene whose gene product interacts with Cyclin B to form the mitosis promoting factor (MPF) , depends essentially on NF-Y , too. Also the Checkpoint kinase Chk2  and the securin gene  are repressed by p53 in a NF-Y dependent manner. This mode of repression has also been demonstrated for the topoisomerase IIα gene [64, 65].
Further transcription factors bound by p53 to induce transcriptional repression are c-myc , CBF , STAT5 , HIF1α, , Estrogen Receptor  and the hepatic nuclear factors (HNF) 4α1 and 6α [71, 72]. Transcriptional repression of target genes is mostly achieved through preventing the activating transcription factors from binding to the respective promoter. It applies to HNF4α1, that p53 also binds directly to the HNF4α1 promoter and represses its activity which was in part relieved by inhibition of HDACs. In addition, p53 binds to the ligand binding domain of HNF4α1 and inhibits its transactivating function. Therefore, the regulation of the HNF4α promoter involves different mechanisms. This illustrates a well-known feature of p53-function: p53 represses its targets often by interfering with their expression on multiple ways. In conclusion, the prevention of transactivators from binding to promoters by p53, either through protein-protein interaction or through competition for DNA binding, is a widespread mechanism which has implications on the expression of numerous genes.
Apart from the interaction with coactivators, different studies have shown that p53 interacts with the basal transcription machinery and thereby represses promoter activity [73–76] (Figure 3, panel II). For some target genes this mechanism was assumed, such as MCL-1 , Bcl-2  and Cox-2 . Binding of p53 to TBP was shown to counteract its association to other components of the basal transcription machinery like TFIIA  and TFIIIB . The functional significance of this interaction of p53 and TBP was elucidated by Crighton et al. . They found that p53 specifically binds to TBP and disrupts TBP-TFIIIB interactions. This prevents TFIIIB from binding to TFIIIC2 which is a critical step for the assembly of transcription machinery on tRNA gene promoters. Additionally, the interaction between TFIIIB and RNA polymerase III is also impaired. Finally, the amount of tRNA is markedly decreased after expression of p53 which is not bound to tRNA promoters thereby raising the question of how specific this repression mechanism is. The authors also showed that TBP promoter occupancy after p53 expression is indeed lower at tRNA promoters but increased on the p21CIP1 promoter region. So, p53-TBP interaction is involved in repression and transactivation as well. Another study, however, showed that p53-TBP interaction is not sufficient for repression of stimulated promoters. p53 was able to repress promoter activity stimulated by different coactivators in Drosophila Schneider cells, but overexpression of TBP did not rescue the inhibition of activated promoters . Therefore, it is likely that there are other, maybe basal, transcription cofactors which are involved in the repression by transcription machinery interference. One proof of this principle was demonstrated by Gridasova and Henry who investigated repression of small nuclear RNAs U1 and U6 by p53 . Intriguingly, they found that p53 is in fact recruited to the snRNA promoters but their repression is not dependent on that interaction. p53 binds to TBP and to snRNA-activating protein complex (SNAPC), a basal transcription factor specific for snRNA genes which binds to proximal sequence elements . Therefore, SNAPC might guide p53 to snRNA promoters and cooperate in the repression mechanism.
3.3. Regulation of Chromatin Structure and Promoter Methylation
Regulation of epigenetic modifications on histones or promoters is a common mechanism for transcriptional control. p53 downregulates target genes by recruitment of histone- and promoter-modifying enzymes or mediators of these. A high number of genes were shown to be regulated, at least in part, by altering chromatin structure rendering the promoter regions inaccessible for the transcription machinery and cofactors (Figure 5). The survivin , PSA , c-myc , AFP [88–91] and HNF4α genes [71, 72] are regulated in part by recruitment of histone deacetylases (HDACs). In addition numerous cell-cycle genes like Cdc2 , Cyclin B2 [31, 34], Microtubule associated protein 4 (Map4) [92, 93], Snk/Plk-akin kinase (SAK) , Mitosis Arrest Deficiency 1 (MAD1)  and Cdc25C  are downregulated by this mechanism. Cdc2 and Cyclin B are important for G2/M progression and their expression levels depend on NF-Y. As described above, p53 was shown to interact with promoter-bound NF-Y. After DNA-damage p53 becomes acetylated at the C-terminal domain and recruits HDAC1, 4 and 5 followed by decreased histone acetylation and promoter repression [31, 34]. In addition to chromatin modification, also promoter methylation by DNMT1 is involved into the repression of the Cdc2 and Cdc25C gene . Chromatin immunoprecipitations of Doxorubicin treated cells showed that the repressor complex which mediates downregulation of promoter activity contains p53, HDAC1, DNMT1 and correlated with dimethylated histone H3 (H3K9me2) but no NF-Y was detected. Furthermore, these promoters displayed internal CpG methylation in the vicinity of the p53 binding sites after DNA-damage and this methylation was dependent on p53 .
Depending on the target gene, the repressor protein complexes assembled may show different components. The repression of the Map4 and MAD1 genes [93, 95], for example, is carried out by a complex including p53, HDACs and the corepressor mSin3a, which was shown to mediate the interaction between p53 and HDAC . mSin3A also plays a pivotal role in the p53-dependent repression of other genes like AFP  and c-myc . Ho et al.  observed that p53-induced G1-arrest is dependent on functional c-myc repression and this regulation is sensitive to the HDAC-inhibitor trichostatin A (TSA). Accordingly, the corepressor mSin3a is recruited on the c-myc promoter possibly for mediating the interaction with HDACs. In conclusion the repressor complexes which control chromatin structure of repressed target genes contain different components depending on the gene to be silenced. p53 is often directly associated with the promoter but may also be bound indirectly through interactions with other promoter-binding proteins.
4. Indirect Repression through Regulation of Other Target Genes
For indirect repression, p53 binding to the promoter or to cofactors is not required (Figure 5). Indeed, p53 regulates expression of numerous transcription factors like TCF4 , ATF3  or c-fos , and changes in their expression influence transcription of their target genes, too (Figure 5, panel I). One example of indirect p53-dependent repression is the regulation of the Cyclin D1 gene by crosstalk with the NF-B pathway . A NF-B p52/Bcl3-complex controls expression from the Cyclin D1 gene and p53 inhibits expression of the Bcl-3 coactivator unit. Interestingly, the p52-subunit was required for Cyclin D1 repression, suggesting that it acts as a repressor when Bcl-3 is absent. The p52-subunit is able to recruit HDAC1 to the Cyclin D1 promoter and interactions of these proteins increase significantly after p53 induction . So, this repression mechanism ultimately culminates in changes in chromatin structure which leads to transcriptional repression.
Special importance to gene repression by p53 has the target gene p21CIP1. This CDK inhibitor protein has been implicated in the repression mechanism of several genes like CHK1 , survivin [36, 39], topoisomerase IIα [102, 103], Cyclin B1 [36, 103–105], Cyclin A2 [36, 103], Cdc2 [36, 103, 104], Cdc25A [106, 107], Cdc25C , hTERT , PLK1 , Rad51 , the MCM genes  and BRCA1 . The p21CIP1 protein was shown to inhibit several CDKs which are essential for cell-cycle progression. Usually, the retinoblastoma protein gets hyperphosphorylated by CDKs in G1/S-Phase of the cell-cycle which leads to release of bound E2F transcription factor complexes mediating transcriptional regulation of numerous genes during the cell-cycle. p21CIP1 causes decreased CDK-activity leading to hypophosphorylated Rb which in turn remains complexed with E2F [111, 112] (Figure 5, panel II). Expression of E2F-dependent genes is therefore decreased as a consequence of p21CIP1 expression. When p21CIP1 is involved in the molecular mechanism, p53-dependent repression at the promoter level should depend on E2F-binding sites, which in fact was demonstrated for survivin , hTERT , Cdc2 [103, 104, 113], Cyclin A  and also suggested for Cyclin B1 [103, 104].
Analyses of the cdc2 promoter showed that its repression is dependent on both p21CIP1 and the retinoblastoma proteins (Rb, p130 and p107). p21CIP1 activity induces a p130/E2F4 complex which is recruited to the CDE/CHR elements of the cdc2 promoter. Mutation of these elements impairs p53-dependent repression . The remaining potential of p53 to repress the promoter independent of the CDE/CHR elements may be attributed to other repression mechanisms, for example the recruitment of chromatin remodelling enzymes with subsequent alterations in the chromatin structure. So cdc2 is another example of a p53-target gene, where many different molecular mechanisms act together for mediation of p53-dependent repression.
Beside cdc2, p53-dependent repression of several genes is considered to rely on the presence of CDE/CHR tandem elements within the respective promoters . Plk1 and topoisomerase IIa genes were shown to be downregulated through CDE⁄CHR sites upon p21CIP1 overexpression . The CDE/CHR tandem element and p21CIP1 are also found to be responsible for mediation of p53-dependent repression of the Cdc20 promoter . However, these results have been challenged by a recent study, demonstrating that under physiological levels of p53, neither p21CIP1 nor the CDE⁄CHR elements are required for p53-dependent repression. Instead, the authors identified a p53-binding element further upstream in the Cdc20 promoter as the major regulatory site .
Nevertheless, consistent with the findings of Kidokoro et al.  is the mechanism of transcriptional repression of G2/M genes after DNA damage postulated by Mannefeld et al., which require functional p21CIP1 . As described above, p21CIP1 inhibits activity of CDK complexes, thereby triggering hypophosphorylation of retinoblastoma proteins and formation of repressive pocket protein-E2F complexes on E2F-dependent promoters. Mannefeld and coworkers found that in response to DNA damage binding of E2F4 and p130 to the recently identified E2F-pocketprotein complex LINC/DREAM is induced and displaces transcription factor B-Myb from this complex. It is postulated, that the switch of LINC/DREAM associated proteins depends on the phosphorylation level of p130 and thereby on p21CIP1 activity. The G2/M genes Cyclin B1, CDC2, BIRC5 and UBCH10 were shown to be regulated by this mechanism . In addition to these G2/M genes participation of E2Fs and pocket proteins, especially p130, in DNA damage-induced inhibition of several other cell-cycle regulators is described [109, 113, 118, 119] suggesting that this mechanism is another widespread mode of p53-dependent repression.
A further example for p21CIP1-dependent downregulation is the telomerase (hTERT) gene. Mutational analyses of the promoter showed that an atypical E2F site in the 5′UTR region is responsible for the repression of this promoter. Cotransfection experiments with a dominant-negative E2F mutant showed that p53 is not able to repress hTERT promoter after inhibition of E2F activity. Furthermore, the knockdown of the Rb, p130 and p107 proteins significantly reduced the p53-mediated repression . Therefore, the p21CIP1-Rb-E2F axis controls repression of the telomerase gene after p53 expression. As the Rb-proteins were shown to recruit HDACs to target gene promoters which is critical for specific gene repression , the hTERT downregulation was investigated after trichostatin treatment. The inhibition of HDACs attenuated the repression effect of p53, indicating that histone deacetylation is involved in the repression of hTERT promoter . As described above, the repression of hTERT was also demonstrated to be dependent on an interaction of p53 with the SP1 coactivator . Again, there are different mechanisms which may act together to repress hTERT gene expression.
The expression of the cell-cycle phosphatase Cdc25A is also repressed by p53 . We observed that Cdc25A repression after DNA-damage is abrogated after p21CIP1-knockdown. In consistence with that we and others found that translation is essential for transcriptional repression. Inhibition of translation via cycloheximide abrogates repression of Cdc25A and other target genes  indicating that other proteins have to be synthesised during p53 expression to take part in the repression mechanism (unpublished results). This suggests contribution of the p21CIP1-Rb-E2F pathway in the p53-dependent repression of Cdc25A. It was also suggested that p21CIP1 directly binds as a transcriptional regulator to Cdc25A promoter DNA . Its presence on DNA is associated with an inhibition of the recruitment of the p300 histone acetylase and with a downregulation of histone H4 acetylation .
In conclusion, gene regulation by indirect mechanisms is a common feature of p53 activity. In particular, p21CIP1 is an important mediator of p53-dependent repression of many target genes, mainly by inhibition of cyclin dependent kinases provoking subsequent interference with the Rb-E2F-pathway.
5. p53-Dependent Repression by Regulation of Noncoding RNAs
In recent years, nonprotein coding RNAs (ncRNA) have emerged as novel regulators of different signalling pathways. Notably, ncRNAs regulate the p53 pathway upstream and downstream of p53. A transcript located antisense to the p53 gene (Wrap53) is induced after DNA damage and directly interacts and stabilizes the p53 mRNA . Interestingly, the p53 mRNA itself acts as a ncRNA by interaction with E3 Ubiquitin Ligase MDM2 . This interaction contributes to inactivation of MDM2-mediated p53 degradation after DNA damage. Together, ncRNAs are an integral component of the p53 signalling network.
Regulation of ncRNAs by p53 has been investigated in detail for a class of small RNAs called miRNAs. Different groups showed that p53 upregulates genes of the miRNA-34 family [124–128]. MicroRNAs are small 21–25 nucleotide long noncoding RNAs which can regulate gene expression posttranscriptionally by interference with mRNA-translation or by induction of mRNA-decay [129, 130]. By proteomic studies it was demonstrated that one miRNA may have hundreds of targets [131, 132]. The p53 mediated repression of gene expression by miRNAs is not carried out at the promoter level but leads to changes in mRNA- and protein-levels. Only recently, it was demonstrated that miRNAs primarily act through induction of mRNA decay and not through translation inhibition alone as anticipated earlier . Interestingly, miRNA-34 induction promotes apoptosis and leads to repression of diverse genes involved in cell-cycle progression, apoptosis, DNA repair, and angiogenesis . The regulation of miRNA-192 and -215 by p53 also influences cell-cycle progression [134, 135]. This function has been attributed, in part, to the miRNA-mediated induction of p21CIP1 in cells after overexpression of miRNA-192 and -215. On the other hand, miRNA-mediated repression of cell-cycle genes may also contribute to cell-cycle arrest. miRNA-107 is transcriptionally induced by p53 and represses CDK6 and the Rb Related 2 (p130) . Overexpression of this miRNA has been shown to induce a G1/S cell-cycle arrest . An interesting aspect of miRNA-107 regulation is that this miRNA also influences the angiogenesis pathway by interfering with HIF1β expression . This shows that p53-dependent regulation of one miRNA may have an impact on different signaling pathways. The miRNA-25, -93, -106b cluster, which is intronic to the MCM7 gene, was shown to be repressed by p53. This repression is mediated by inhibition of E2F1 activity, which controls expression of the host gene MCM7. Overexpression of these miRNAs promotes cell proliferation and delays senescence . Another example is the proto-oncogene c-myc which is repressed by different p53-dependent mechanisms. In one of these pathways p53 transcriptionally induces miRNA-145 which then represses c-myc expression . In addition, c-myc is regulated by interference of p53 with components of the basal transcription machinery, as described above . miRNA genes are also downregulated by this mechanism. The miR-17-92 cluster is transcriptionally downregulated under hypoxic conditions. p53 binds at a response element in the proximal promoter region in which this site overlaps with the TBP binding site. Thus, p53 displaces TBP at the miR-17-92 cluster promoter to repress transcription. Lower expression of these miRNAs assists in hypoxia mediated p53-dependent apoptosis induction .
In contrast to miRNAs, long noncoding RNAs are a heterogeneous class of transcripts which have diverse functions in different processes. Genome wide investigation of RNA expression and chromatin modifications has shown that there are over a thousand long intergenic ncRNAs (lincRNAs) in the human genome [142, 143]. A subset of these lincRNAs is regulated by p53. Only recently, lincRNA-p21 was discovered as a global regulator of p53-dependent repression in mouse. This RNA interacts with the heterogeneous nuclear ribonucleoprotein K (hnRNP-K) and is needed for recruitment of this complex to specific genomic sites of repressed genes . Knock down of lincRNA-p21 prevented p53-dependent repression of many genes and also attenuated induction of apoptosis. These results show that regulated noncoding RNAs also have a great impact on gene repression by p53. It can be assumed that in the near future a multitude of other ncRNAs, which are involved in the p53-pathway, will be discovered.
Several genome wide expression analyses show that repressed genes make up a large proportion of p53-regulated targets [16, 121]. For example, 5-FU treatment of HCT116 cells results in p53-dependent regulation of 230 of 19,000 genes screened, only 41 of them were activated but 189 were repressed . Results of another study suggest, that p53-induced repression plays a decisive role in cell-cycle control, as nearly half of repressed p53 targets are cell-cycle related . However, genes affected by p53-mediated repression cover a wide range of cellular functions . In addition to cell-cycle control and induction of apoptosis, metabolism, infection and immune response, adhesion, DNA replication and repair, cytoskeleton organization and cell signalling are affected . However, the observed spectrum of target genes depends on the stimulus used .
As demonstrated here, the mechanisms leading to p53-dependent transcriptional repression are diverse. Generally, in contrast to activation there are many ways of p53-dependent repression. Direct mechanisms of target gene repression comprise DNA-binding-dependent mechanisms and DNA-binding independent mechanisms. In these cases, p53 acts directly on the target gene promoter by displacement of coactivators or general transcription factors. p53 can also be bound to the promoter through interaction with other DNA-binding proteins. A lot of target gene promoters are downregulated by changes in chromatin structure which leads to inaccessibility of the promoter region. The second large class of repression mechanisms comprises the indirect modes, that is, downregulation of promoter activity where p53-interactions at the target gene promoters are completely absent. Particularly, the relevance of the p53 target gene p21CIP1 for this mechanism is often published. p21CIP1 is thought to contribute to the p53-dependent repression mainly by inhibition of cyclin dependent kinases provoking subsequent interference with the Rb-E2F-pathway. In many cases there is more than one repression mechanism influencing the promoter activity of a target gene. It is likely that different mechanisms work synergistically together and that they may also compensate for each other.
This is an intriguing difference to p53-dependent transactivation which is carried out in most cases by a similar mechanism. Why are there such diverse repression mechanisms? First of all, this diversity may contribute to a higher flexibility in terms of cell type and tissue-dependent regulation of target genes after cellular stress. p53 up- and downregulates different target genes in different tissues which leads to different physiological outcomes . These tissue-specific adjustments of the p53 signalling pathway are achieved through modulation or binding to tissue-specific coactivators (e.g., liver specific downregulation of the AFP gene). Another reason for such a diversity of repression mechanisms may be the enhanced robustness in the signalling network in response to cellular stress. Aberrant changes in expression or mutation of single components of these pathways might have only a minor impact on the stability of the whole system. However, mutation of p53 is widely observed in different tumors and some mutants may have even oncogenic functions. In conclusion, care has to be taken in respect to the different cell systems used for investigations, as this may have influences on the repression mechanisms. The heterogeneity and the mechanistic diversity of transcriptional repression is an interesting feature of p53 function making it impossible to assign one mechanism to one target gene. However, up to date there are a lot of other candidate genes that are repressed by p53, including SDF-1 , Interleukin-2 and Interleukin-4 , Interleukin-6 , stathmin and FKBP25 , PCNA , MGMT , Ets1 and Ets2 , Cks1 , Cks2 , BRCA1 [156, 157], PIK3CA , hGR1 , p202 , RECQ4 , Clusterin , PIQ , TCF4 , and HMMR (RHAMM) , but the detailed mechanisms of their regulation are not yet clear. As expression of p53 leads to cell-cycle changes it is difficult to determine for some genes whether their regulation is the cause or the consequence of cell-cycle arrest. As outlined above, several cell-cycle genes are efficiently repressed by p53 and their expression pattern might additionally be influenced by cell-cycle arrest.
In addition to proteins, noncoding RNAs have evolved as important components of the p53 network. Many miRNAs are regulated by p53 and may have an impact on hundreds of other proteins in the cell. The mechanisms leading to transcriptional repression mediated by long noncoding RNAs are only beginning to emerge. Many of them interact with chromatin modifying complexes and target them to specific sites in the genome. p53-induced lincRNA-p21 recruits hnRNP-K to genes which are repressed in a p53-dependent manner and this pathway is important for apoptosis induction. To date, the underlying mechanisms of this regulation pathway for specific targets are not clear and more research is needed in order to elucidate the contribution of many other ncRNAs to the p53-dependent gene regulation.
The knowledge of repressed target genes and the complexity of the signalling pathways leading to transcriptional downregulation is rapidly expanding due to new data provided by genome-wide transcriptome profiling and ChIP-Seq methods. More insights into the different mechanisms may enable the researchers in the future to predict repression mechanisms on the basis of promoter sequences. The elucidation of the complete p53-“regulome” is important for our understanding of its tumor suppressor function and understanding its mechanisms of gene regulation represent also the key for therapeutic approaches like the reconstitution of wild-type functions in tumors where p53 was mutated.
The authors would like to thank Nathaniel Saidu and Mathias Montenarh for critically reading the paper. This work was supported by a formel.1 junior research grant at the University of Leipzig (to L. Böhlig). L. Böhlig and K. Rother contributed equally to this work.
- F. Talos and U. M. Moll, “Role of the p53 family in stabilizing the genome and preventing polyploidization,” Advances in Experimental Medicine and Biology, vol. 676, pp. 73–91, 2010.
- D. P. Lane and L. V. Crawford, “T antigen is bound to a host protein in SV40 transformed cells,” Nature, vol. 278, no. 5701, pp. 261–263, 1979.
- D. I. H. Linzer and A. J. Levine, “Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40 transformed cells and uninfected embryonal carcinoma cells,” Cell, vol. 17, no. 1, pp. 43–52, 1979.
- B. Vogelstein, D. Lane, and A. J. Levine, “Surfing the p53 network,” Nature, vol. 408, no. 6810, pp. 307–310, 2000.
- L. A. Donehower, M. Harvey, B. L. Slagle et al., “Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours,” Nature, vol. 356, no. 6366, pp. 215–221, 1992.
- A. Ventura, D. G. Kirsch, M. E. McLaughlin et al., “Restoration of p53 function leads to tumour regression in vivo,” Nature, vol. 445, no. 7128, pp. 661–665, 2007.
- W. Xue, L. Zender, C. Miething et al., “Senescence and tumour clearance is triggered by p53 restoration in murine liver carcinomas,” Nature, vol. 445, no. 7128, pp. 656–660, 2007.
- W. S. El-Deiry, S. E. Kern, J. A. Pietenpol, K. W. Kinzler, and B. Vogelstein, “Definition of a consensus binding site for p53,” Nature Genetics, vol. 1, no. 1, pp. 45–49, 1992.
- G. Farmer, J. Colgan, Y. Nakatani, J. L. Manley, and C. Prives, “Functional interaction between p53, the TATA-binding protein (TBP), and TBP-associated factors in vivo,” Molecular and Cellular Biology, vol. 16, no. 8, pp. 4295–4304, 1996.
- X. Liu, C. W. Miller, P. H. Koeffler, and A. J. Berk, “The p53 activation domain binds the TATA box-binding polypeptide in holo-TFIID, and a neighboring p53 domain inhibits transcription,” Molecular and Cellular Biology, vol. 13, no. 6, pp. 3291–3300, 1993.
- H. Lu and A. J. Levine, “Human TAF(II)31 protein is a transcriptional coactivator of the p53 protein,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 11, pp. 5154–5158, 1995.
- T. Miyashita and J. C. Reed, “Tumor suppressor p53 is a direct transcriptional activator of the human bax gene,” Cell, vol. 80, no. 2, pp. 293–299, 1995.
- W. S. El-Deiry, T. Tokino, V. E. Velculescu et al., “WAF1, a potential mediator of p53 tumor suppression,” Cell, vol. 75, no. 4, pp. 817–825, 1993.
- H. Hermeking, C. Lengauer, K. Polyak et al., “14-3-3σ is a p53-regulated inhibitor of G2/M progression,” Molecular Cell, vol. 1, no. 1, pp. 3–11, 1997.
- J. Ho and S. Benchimol, “Transcriptional repression mediated by the p53 tumour suppressor,” Cell Death and Differentiation, vol. 10, no. 4, pp. 404–408, 2003.
- C. Koumenis, R. Alarcon, E. Hammond et al., “Regulation of p53 by hypoxia: dissociation of transcriptional repression and apoptosis from p53-dependent transactivation,” Molecular and Cellular Biology, vol. 21, no. 4, pp. 1297–1310, 2001.
- T. Riley, E. Sontag, P. Chen, and A. Levine, “Transcriptional control of human p53-regulated genes,” Nature Reviews Molecular Cell Biology, vol. 9, no. 5, pp. 402–412, 2008.
- M. Shi, D. Liu, B. Shen, and N. Guo, “Helpers of the cellular gatekeeper-miRNAs dance in P53 network,” Biochimica et Biophysica Acta, vol. 1805, no. 2, pp. 218–225, 2010.
- K. H. Vousden and C. Prives, “Blinded by the light: the growing complexity of p53,” Cell, vol. 137, no. 3, pp. 413–431, 2009.
- D. Menendez, A. Inga, and M. A. Resnick, “The expanding universe of p53 targets,” Nature Reviews Cancer, vol. 9, no. 10, pp. 724–737, 2009.
- B. Wang, Z. Xiao, and EE. C. Ren, “Redefining the p53 response element,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 34, pp. 14373–14378, 2009.
- R. A. Johnson, T. A. Ince, and K. W. Scotto, “Transcriptional repression by p53 through direct binding to a novel DNA element,” Journal of Biological Chemistry, vol. 276, no. 29, pp. 27716–27720, 2001.
- S. Godar, T. A. Ince, G. W. Bell et al., “Growth-inhibitory and tumor-suppressive functions of p53 depend on its repression of CD44 expression,” Cell, vol. 134, no. 1, pp. 62–73, 2008.
- J. Marks, Z. Saifudeen, S. Dipp, and S. S. El-Dahr, “Two functionally divergent p53-responsive elements in the rat bradykinin B2 receptor promoter,” Journal of Biological Chemistry, vol. 278, no. 36, pp. 34158–34166, 2003.
- K. C. Lee, A. J. Crowe, and M. C. Barton, “p53-mediated repression of alpha-fetoprotein gene expression by specific DNA binding,” Molecular and Cellular Biology, vol. 19, no. 2, pp. 1279–1288, 1999.
- D. S. Wilkinson, W. W. Tsai, M. A. Schumacher, and M. C. Barton, “Chromatin-bound p53 anchors activated Smads and the mSin3A corepressor to confer transforming growth factor β-mediated transcription repression,” Molecular and Cellular Biology, vol. 28, no. 6, pp. 1988–1998, 2008.
- D. van Bodegom, W. Roessingh, A. Pridjian, and S. S. El Dahr, “Mechanisms of p53-mediated repression of the human polycystic kidney disease-1 promoter,” Biochimica et Biophysica Acta, vol. 1799, no. 7, pp. 502–509, 2010.
- A. Zaky, C. Busso, T. Izumi et al., “Regulation of the human AP-endonuclease (APE1/Ref-1) expression by the tumor suppressor p53 in response to DNA damage,” Nucleic Acids Research, vol. 36, no. 5, pp. 1555–1566, 2008.
- S. A. Innocente and J. M. Lee, “p53 is a NF-Y- and p21-independent, Sp1-dependent repressor of cyclin B1 transcription,” FEBS Letters, vol. 579, no. 5, pp. 1001–1007, 2005.
- S. ST. Clair, L. Giono, S. Varmeh-Ziaie et al., “DNA damage-induced downregulation of Cdc25C is mediated by p53 via two independent mechanisms: one involves direct binding to the cdc25C promoter,” Molecular Cell, vol. 16, no. 5, pp. 725–736, 2004.
- C. Imbriano, A. Gurtner, F. Cocchiarella et al., “Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G/M promoters,” Molecular and Cellular Biology, vol. 25, no. 9, pp. 3737–3751, 2005.
- K. Krause, U. Haugwitz, M. Wasner, M. Wiedmann, J. Mössner, and K. Engeland, “Expression of the cell cycle phosphatase cdc25C is down-regulated by the tumor suppressor protein p53 but not by p73,” Biochemical and Biophysical Research Communications, vol. 284, no. 3, pp. 743–750, 2001.
- I. Manni, G. Mazzaro, A. Gurtner et al., “NF-Y mediates the transcriptional inhibition of the cyclin B1, cyclin B2, and cdc25C promoters upon induced G arrest,” Journal of Biological Chemistry, vol. 276, no. 8, pp. 5570–5576, 2001.
- V. Basile, R. Mantovani, and C. Imbriano, “DNA damage promotes histone deacetylase 4 nuclear localization and repression of G/M promoters, via p53 C-terminal lysines,” Journal of Biological Chemistry, vol. 281, no. 4, pp. 2347–2357, 2006.
- G. Le Gac, P. O. Estève, C. Ferec, and S. Pradhan, “DNA damage-induced down-regulation of human Cdc25C and Cdc2 is mediated by cooperation between p53 and maintenance DNA (cytosine-5) methyltransferase,” Journal of Biological Chemistry, vol. 281, no. 34, pp. 24161–24170, 2006.
- K. Löhr, C. Möritz, A. Contente, and M. Dobbelstein, “p21/CDKN1A mediates negative regulation of transcription by p53,” Journal of Biological Chemistry, vol. 278, no. 35, pp. 32507–32516, 2003.
- J. Bargonetti, A. Chicas, D. White, and C. Prives, “p53 represses Sp1 DNA binding and HIV-LTR directed transcription,” Cellular and Molecular Biology, vol. 43, no. 7, pp. 935–949, 1997.
- W. H. Hoffman, S. Biade, J. T. Zilfou, J. Chen, and M. Murphy, “Transcriptional repression of the anti-apoptotic survivin gene by wild type p53,” Journal of Biological Chemistry, vol. 277, no. 5, pp. 3247–3257, 2002.
- D. Raj, T. Liu, G. Samadashwily, F. Li, and D. Grossman, “Survivin repression by p53, Rb and E2F2 in normal human melanocytes,” Carcinogenesis, vol. 29, no. 1, pp. 194–201, 2008.
- Y. Jiang, H. I. Saavedra, M. P. Holloway, G. Leone, and R. A. Altura, “Aberrant regulation of survivin by the RB/E2F family of proteins,” Journal of Biological Chemistry, vol. 279, no. 39, pp. 40511–40520, 2004.
- P. Dasgupta, R. Kinkade, B. Joshi, C. DeCook, E. Haura, and S. Chellappan, “Nicotine inhibits apoptosis induced by chemotherapeutic drugs by up-regulating XIAP and survivin,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 16, pp. 6332–6337, 2006.
- N. H. Nabilsi, R. R. Broaddus, and D. S. Loose, “DNA methylation inhibits p53-mediated survivin repression,” Oncogene, vol. 28, no. 19, pp. 2046–2050, 2009.
- S. D'Souza, H. Xin, S. Walter, and D. Choubey, “The gene encoding p202, an interferon-inducible negative regulator of the p53 tumor suppressor, is a target of p53-mediated transcriptional repression,” Journal of Biological Chemistry, vol. 276, no. 1, pp. 298–305, 2001.
- P. S. Kho, Z. Wang, L. Zhuang et al., “p53-regulated transcriptional program associated with genotoxic stress-induced apoptosis,” Journal of Biological Chemistry, vol. 279, no. 20, pp. 21183–21192, 2004.
- C. Li, M. Lin, and J. Liu, “Identification of PRC1 as the p53 target gene uncovers a novel function of p53 in the regulation of cytokinesis,” Oncogene, vol. 23, no. 58, pp. 9336–9347, 2004.
- C. Arias-Lopez, I. Lazaro-Trueba, P. Kerr et al., “p53 modulates homologous recombination by transcriptional regulation of the RAD51 gene,” EMBO Reports, vol. 7, no. 2, pp. 219–224, 2006.
- I. Lazaro-Trueba, C. Arias, and A. Silva, “Double bolt regulation of Rad51 by p53: a role for transcriptional repression,” Cell Cycle, vol. 5, no. 10, pp. 1062–1065, 2006.
- W. J. Hsieh, S. C. Hsieh, C. C. Chen, and F. F. Wang, “Human DDA3 is an oncoprotein down-regulated by p53 and DNA damage,” Biochemical and Biophysical Research Communications, vol. 369, no. 2, pp. 567–572, 2008.
- G. M. Habib, “p53 regulates Hsp90β during arsenite-induced cytotoxicity in glutathione-deficient cells,” Archives of Biochemistry and Biophysics, vol. 481, no. 1, pp. 101–109, 2009.
- B. Wang, P. Feng, Z. Xiao, and EE. C. Ren, “LIM and SH3 protein 1 (Lasp1) is a novel p53 transcriptional target involved in hepatocellular carcinoma,” Journal of Hepatology, vol. 50, no. 3, pp. 528–537, 2009.
- D. Xu, Q. Wang, A. Gruber et al., “Downregulation of telomerase reverse transcriptase mRNA expression by wild type p53 in human tumor cells,” Oncogene, vol. 19, no. 45, pp. 5123–5133, 2000.
- N. J. G. Webster, J. L. Resnik, D. B. Reichart, B. Strauss, M. Haas, and B. L. Seely, “Repression of the insulin receptor promoter by the tumor suppressor gene product p53: a possible mechanism for receptor overexpression in breast cancer,” Cancer Research, vol. 56, no. 12, pp. 2781–2788, 1996.
- C. Ohlsson, N. Kley, H. Werner, and D. Leroith, “p53 regulates insulin-like growth factor-I (IGF-I) receptor expression and IGF-I-induced tyrosine phosphorylation in an osteosarcoma cell line: interaction between p53 and Sp1,” Endocrinology, vol. 139, no. 3, pp. 1101–1107, 1998.
- L. Zhang, D. Yu, M. Hu et al., “Wild-type p53 suppresses angiogenesis in human leiomyosarcoma and synovial sarcoma by transcriptional suppression of vascular endothelial growth factor expression,” Cancer Research, vol. 60, no. 13, pp. 3655–3661, 2000.
- T. Suehiro, T. Kaneda, Y. Ikeda, K. Ar II, Y. Kumon, and K. Hashimoto, “Regulation of human glucocorticoid receptor gene transcription by Sp1 and p53,” Molecular and Cellular Endocrinology, vol. 222, no. 1-2, pp. 33–40, 2004.
- Y. Sun, Y. Sun, L. Wenger, J. L. Rutter, C. E. Brinckerhoff, and H. S. Cheung, “p53 down-regulates human matrix metalloproteinase-1 (collagenase-1) gene expression,” Journal of Biological Chemistry, vol. 274, no. 17, pp. 11535–11540, 1999.
- Y. Sun, X. R. Zeng, L. Wenger, G. S. Firestein, and H. S. Cheung, “p53 down-regulates matrix metalloproteinase-1 by targeting the communications between AP-1 and the basal transcription complex,” Journal of Cellular Biochemistry, vol. 92, no. 2, pp. 258–269, 2004.
- K. Krause, M. Wasner, W. Reinhard et al., “The tumour suppressor protein p53 can repress transcription of cyclin B,” Nucleic Acids Research, vol. 28, no. 22, pp. 4410–4418, 2000.
- M. S. Jung, J. Yun, H. D. Chae et al., “p53 and its homologues, p63 and p73, induce a replicative senescence through inactivation of NF-Y transcription factor,” Oncogene, vol. 20, no. 41, pp. 5818–5825, 2001.
- J. C. Labbe, M. G. Lee, P. Nurse, A. Picard, and M. Doree, “Activation at M-phase of a protein kinase encoded by a starfish homologue of the cell cycle control gene cdc2,” Nature, vol. 335, no. 6187, pp. 251–254, 1988.
- J. Yun, H. D. Chae, H. E. Choy et al., “p53 negatively regulates cdc2 transcription via the CCAAT-binding NF-Y transcription factor,” Journal of Biological Chemistry, vol. 274, no. 42, pp. 29677–29682, 1999.
- T. Matsui, Y. Katsuno, T. Inoue et al., “Negative regulation of Chk2 expression by p53 is dependent on the CCAAT-binding transcription factor NF-Y,” Journal of Biological Chemistry, vol. 279, no. 24, pp. 25093–25100, 2004.
- Y. Zhou, K. R. Mehta, A. P. Choi, S. Scolavino, and X. Zhang, “DNA damage-induced inhibition of securin expression is mediated by p53,” Journal of Biological Chemistry, vol. 278, no. 1, pp. 462–470, 2003.
- A. A. Joshi, Z. Wu, R. F. Reed, and D. P. Suttle, “Nuclear factor-Y binding to the topoisomerase IIα promoter is inhibited by both the p53 tumor suppressor and anticancer drugs,” Molecular Pharmacology, vol. 63, no. 2, pp. 359–367, 2003.
- Q. Wang, G. P. Zambetti, and D. P. Suttle, “Inhibition of DNA topoisomerase IIα gene expression by the p53 tumor suppressor,” Molecular and Cellular Biology, vol. 17, no. 1, pp. 389–397, 1997.
- N. Zhu, L. Gu, H. W. Findley, and M. Zhou, “Transcriptional repression of the eukaryotic initiation factor 4E gene by wild type p53,” Biochemical and Biophysical Research Communications, vol. 335, no. 4, pp. 1272–1279, 2005.
- S. N. Agoff, J. Hou, D. I. H. Linzer, and B. Wu, “Regulation of the human hsp70 promoter by p53,” Science, vol. 259, no. 5091, pp. 84–87, 1993.
- M. Fritsche, M. Mundt, C. Merkle, R. Jähne, and B. Groner, “P53 suppresses cytokine induced, Stat5 mediated activation of transcription,” Molecular and Cellular Endocrinology, vol. 143, no. 1-2, pp. 143–154, 1998.
- M. V. Blagosklonny, W. G. An, L. Y. Romanova, J. Trepel, T. Fojo, and L. Neckers, “p53 inhibits hypoxia-inducible factor-stimulated transcription,” Journal of Biological Chemistry, vol. 273, no. 20, pp. 11995–11998, 1998.
- C. L. Yu, P. Driggers, G. Barrera-Hernandez, S. B. Nunez, J. H. Segars, and S. Y. Cheng, “The tumor suppressor p53 is a negative regulator of estrogen receptor signaling pathways,” Biochemical and Biophysical Research Communications, vol. 239, no. 2, pp. 617–620, 1997.
- Y. Maeda, S. D. Seidel, G. Wei, X. Liu, and F. M. Sladek, “Repression of hepatocyte nuclear factor 4α by tumor suppressor p53: involvement of the ligand-binding domain and histone deacetylase activity,” Molecular Endocrinology, vol. 16, no. 2, pp. 402–410, 2002.
- Y. Maeda, W. W. Hwang-Verslues, G. Wei et al., “Tumour suppressor p53 down-regulates the expression of the human hepatocyte nuclear factor 4α (HNF4α) gene,” Biochemical Journal, vol. 400, no. 2, pp. 303–313, 2006.
- N. Horikoshi, A. Usheva, J. Chen, A. J. Levine, R. Weinmann, and T. Shenk, “Two domains of p53 interact with the TATA-binding protein, and the adenovirus 13S E1A protein disrupts the association, relieving p53-mediated transcriptional repression,” Molecular and Cellular Biology, vol. 15, no. 1, pp. 227–234, 1995.
- D. H. Mack, J. Vartikar, J. M. Pipas, and L. A. Laimins, “Specific repression of TATA-mediated but not initiator-mediated transcription by wild-type p53,” Nature, vol. 363, no. 6426, pp. 281–283, 1993.
- E. Seto, A. Usheva, G. P. Zambetti et al., “Wild-type p53 binds to the TATA-binding protein and represses transcription,” Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 24, pp. 12028–12032, 1992.
- R. Truant, H. Xiao, C. J. Ingles, and J. Greenblatt, “Direct interaction between the transcriptional activation domain of human p53 and the TATA box-binding protein,” Journal of Biological Chemistry, vol. 268, no. 4, pp. 2284–2287, 1993.
- M. Pietrzak and M. Puzianowska-Kuznicka, “p53-dependent repression of the human MCL-1 gene encoding an anti-apoptotic member of the BCL-2 family: the role of Sp1 and of basic transcription factor binding sites in the MCL-1 promoter,” Biological Chemistry, vol. 389, no. 4, pp. 383–393, 2008.
- Y. Wu, J. W. Mehew, C. A. Heckman, M. Arcinas, and L. M. Boxer, “Negative regulation of bcl-2 expression by p53 in hematopoietic cells,” Oncogene, vol. 20, no. 2, pp. 240–251, 2001.
- K. Subbaramaiah, N. Altorkil, W. J. Chung, J. R. Mestre, A. Sampat, and A. J. Dannenberg, “Inhibition of cyclooxygenase-2 gene expression by p53,” Journal of Biological Chemistry, vol. 274, no. 16, pp. 10911–10915, 1999.
- N. Ragimov, A. Krauskopf, N. Navot, V. Rotter, M. Oren, and Y. Aloni, “Wild-type but not mutant p53 can repress transcription initiation in vitro by interfering with the binding of basal transcription factors to the TATA motif,” Oncogene, vol. 8, no. 5, pp. 1183–1193, 1993.
- D. Crighton, A. Woiwode, C. Zhang et al., “p53 represses RNA polymerase III transcription by targeting TBP and inhibiting promoter occupancy by TFIIIB,” EMBO Journal, vol. 22, no. 11, pp. 2810–2820, 2003.
- G. Farmer, P. Friedlander, J. Colgan, J. L. Manley, and C. Prives, “Transcriptional repression by p53 involves molecular interactions distinct from those with the TATA box binding protein,” Nucleic Acids Research, vol. 24, no. 21, pp. 4281–4288, 1996.
- A. A. Gridasova and R. W. Henry, “The p53 tumor suppressor protein represses human snRNA gene transcription by RNA polymerases II and III independently of sequence-specific DNA binding,” Molecular and Cellular Biology, vol. 25, no. 8, pp. 3247–3260, 2005.
- C. L. Sadowski, R. W. Henry, S. M. Lobo, and N. Hernandez, “Targeting TBP to a non-TATA box cis-regulatory element: a TBP-containing complex activates transcription from snRNA promoters through the PSE,” Genes and Development, vol. 7, no. 8, pp. 1535–1548, 1993.
- A. Mirza, M. McGuirk, T. N. Hockenberry et al., “Human survivin is negatively regulated by wild-type p53 and participates in p53-dependent apoptotic pathway,” Oncogene, vol. 21, no. 17, pp. 2613–2622, 2002.
- K. V. Gurova, O. W. Roklin, V. I. Krivokrysenko et al., “Expression of prostate specific antigen (PSA) is negatively regulated by p53,” Oncogene, vol. 21, no. 1, pp. 153–157, 2002.
- J. S. L. Ho, W. Ma, D. Y. L. Mao, and S. Benchimol, “p53-dependent transcriptional repression of c-myc is required for G cell cycle arrest,” Molecular and Cellular Biology, vol. 25, no. 17, pp. 7423–7431, 2005.
- S. K. Ogden, K. C. Lee, K. Wernke-Dollries, S. A. Stratton, B. Aronow, and M. C. Barton, “p53 targets chromatin structure alteration to repress alpha-fetoprotein gene expression,” Journal of Biological Chemistry, vol. 276, no. 45, pp. 42057–42062, 2001.
- R. Cui, T. T. Nguyen, J. H. Taube, S. A. Stratton, M. H. Feuerman, and M. C. Barton, “Family members p53 and p73 act together in chromatin modification and direct repression of α-fetoprotein transcription,” Journal of Biological Chemistry, vol. 280, no. 47, pp. 39152–39160, 2005.
- T. T. Nguyen, K. Cho, S. A. Stratton, and M. C. Barton, “Transcription factor interactions and chromatin modifications associated with p53-mediated, developmental repression of the alpha-fetoprotein gene,” Molecular and Cellular Biology, vol. 25, no. 6, pp. 2147–2157, 2005.
- D. S. Wilkinson, S. K. Ogden, S. A. Stratton et al., “A direct intersection between p53 and transforming growth factor β pathways targets chromatin modification and transcription repression of the α-fetoprotein gene,” Molecular and Cellular Biology, vol. 25, no. 3, pp. 1200–1212, 2005.
- M. Murphy, J. Ahn, K. K. Walker et al., “Transcriptional repression by wild-type p53 utilizes histone deacetylases, mediated by interaction with mSin3a,” Genes and Development, vol. 13, no. 19, pp. 2490–2501, 1999.
- M. Murphy, A. Hinman, and A. J. Levine, “Wild-type p53 negatively regulates the expression of a microtubule-associated protein,” Genes and Development, vol. 10, no. 23, pp. 2971–2980, 1996.
- J. Li, M. Tan, L. Li, D. Pamarthy, T. S. Lawrence, and Y. Sun, “SAK, a new polo-like kinase, is transcriptionally repressed by p53 and induces apoptosis upon RNAi silencing,” Neoplasia, vol. 7, no. 4, pp. 312–323, 2005.
- A. C. S. Chun and D. Y. Jin, “Transcriptional regulation of mitotic checkpoint gene MAD1 by p53,” Journal of Biological Chemistry, vol. 278, no. 39, pp. 37439–37450, 2003.
- P. O. Estève, H. G. Chin, and S. Pradhan, “Human maintenance DNA (cytosine-5)-methyltransferase and p53 modulate expression of p53-repressed promoters,” Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 4, pp. 1000–1005, 2005.
- K. Rother, C. Johne, K. Spiesbach et al., “Identification of Tcf-4 as a transcriptional target of p53 signalling,” Oncogene, vol. 23, no. 19, pp. 3376–3384, 2004.
- S. A. Amundson, M. Bittner, Y. Chen, J. Trent, P. Meltzer, and A. J. Fornace, “Fluorescent cDNA microarray hybridization reveals complexity and heterogeneity of cellular genotoxic stress responses,” Oncogene, vol. 18, no. 24, pp. 3666–3672, 1999.
- D. Ginsberg, F. Mechta, M. Yaniv, and M. Oren, “Wild-type p53 can down-modulate the activity of various promoters,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 22, pp. 9979–9983, 1991.
- S. Rocha, A. M. Martin, D. W. Meek, and N. D. Perkins, “p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-κB subunit with histone deacetylase 1,” Molecular and Cellular Biology, vol. 23, no. 13, pp. 4713–4727, 2003.
- V. Gottifredi, O. Karni-Schmidt, S. Y. Shieh, and C. Prives, “p53 down-regulates CHK1 through p21 and the retinoblastoma protein,” Molecular and Cellular Biology, vol. 21, no. 4, pp. 1066–1076, 2001.
- H. Zhu, B. D. Chang, T. Uchiumi, and I. B. Roninson, “Identification of promoter elements responsible for transcriptional inhibition of polo-like kinase 1 and topoisomerase IIalpha genes by p21(WAF1/CIP1/SDI1),” Cell Cycle, vol. 1, no. 1, pp. 59–66, 2002.
- S. M. De Toledo, E. I. Azzam, P. Keng, S. Laffrenier, and J. B. Little, “Regulation by ionizing radiation of CDC2, cyclin A, cyclin B, Thymidine Kinace, Topoisomerase IIα, and RAD51 expression in normal human diploid fibroblasts is dependent on p53/p21(Waf1),” Cell Growth and Differentiation, vol. 9, no. 11, pp. 887–896, 1998.
- P. M. Flatt, L. J. Tang, C. D. Scatena, S. T. Szak, and J. A. Pietenpol, “p53 regulation of G checkpoint is retinoblastoma protein dependent,” Molecular and Cellular Biology, vol. 20, no. 12, pp. 4210–4223, 2000.
- M. Mannefeld, E. Klassen, and S. Gaubatz, “B-MYB is required for recovery from the DNA damage-induced G checkpoint in p53 mutant cells,” Cancer Research, vol. 69, no. 9, pp. 4073–4080, 2009.
- K. Rother, R. Kirschner, K. Sänger, L. Böhlig, J. Mössner, and K. Engeland, “p53 downregulates expression of the G/S cell cycle phosphatase Cdc25A,” Oncogene, vol. 26, no. 13, pp. 1949–1953, 2007.
- A. Vigneron, J. Cherier, B. Barré, E. Gamelin, and O. Coqueret, “The cell cycle inhibitor p21 binds to the myc and cdc25A promoters upon DNA damage and induces transcriptional repression,” Journal of Biological Chemistry, vol. 281, no. 46, pp. 34742–34750, 2006.
- I. Shats, M. Milyavsky, X. Tang et al., “p53-dependent down-regulation of telomerase is mediated by p21,” Journal of Biological Chemistry, vol. 279, no. 49, pp. 50976–50985, 2004.
- M. W. Jackson, M. K. Agarwal, J. Yang et al., “p130/p107/p105Rb-dependent transcriptional repression during DNA-damage-induced cell-cycle exit at G2,” Journal of Cell Science, vol. 118, no. 9, pp. 1821–1832, 2005.
- M. J. Scian, E. H. Carchman, L. Mohanraj et al., “Wild-type p53 and p73 negatively regulate expression of proliferation related genes,” Oncogene, vol. 27, no. 18, pp. 2583–2593, 2008.
- J. W. Harper, G. R. Adami, N. Wei, K. Keyomarsi, and S. J. Elledge, “The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases,” Cell, vol. 75, no. 4, pp. 805–816, 1993.
- S. W. Hiebert, S. P. Chellappan, J. M. Horowitz, and J. R. Nevins, “The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F,” Genes and Development, vol. 6, no. 2, pp. 177–185, 1992.
- W. R. Taylor, A. H. Schönthal, J. Galante, and G. R. Stark, “p130/E2F4 binds to and represses the cdc2 promoter in response to p53,” Journal of Biological Chemistry, vol. 276, no. 3, pp. 1998–2006, 2001.
- D. Spitkovsky, A. Schulze, B. Boye, and P. Jansen-Dürr, “Down-regulation of Cyclin A gene expression upon genotoxic stress correlates with reduced binding of free E2F to the promoter,” Cell Growth and Differentiation, vol. 8, no. 6, pp. 699–710, 1997.
- G. A. Müller and K. Engeland, “The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription: review article,” FEBS Journal, vol. 277, no. 4, pp. 877–893, 2010.
- T. Kidokoro, C. Tanikawa, Y. Furukawa, T. Katagiri, Y. Nakamura, and K. Matsuda, “CDC20, a potential cancer therapeutic target, is negatively regulated by p53,” Oncogene, vol. 27, no. 11, pp. 1562–1571, 2008.
- T. Banerjee, S. Nath, and S. Roychoudhury, “DNA damage induced p53 downregulates Cdc20 by direct binding to its promoter causing chromatin remodeling,” Nucleic Acids Research, vol. 37, no. 8, pp. 2688–2698, 2009.
- J. G. Jackson and O. M. Pereira-Smith, “Primary and compensatory roles for RB family members at cell cycle gene promoters that are deacetylated and downregulated in doxorubicin-induced senescence of breast cancer cells,” Molecular and Cellular Biology, vol. 26, no. 7, pp. 2501–2510, 2006.
- S. Polager and D. Ginsberg, “E2F mediates sustained G arrest and down-regulation of Stathmin and AIM-1 expression in response to genotoxic stress,” Journal of Biological Chemistry, vol. 278, no. 3, pp. 1443–1449, 2003.
- H. Siddiqui, D. A. Solomon, R. W. Gunawardena, Y. Wang, and E. S. Knudsen, “Histone deacetylation of RB-responsive promoters: requisite for specific gene repression but dispensable for cell cycle inhibition,” Molecular and Cellular Biology, vol. 23, no. 21, pp. 7719–7731, 2003.
- K. B. Spurgers, D. L. Gold, K. R. Coombes et al., “Identification of cell cycle regulatory genes as principal targets of p53-mediated transcriptional repression,” Journal of Biological Chemistry, vol. 281, no. 35, pp. 25134–25142, 2006.
- S. Mahmoudi, S. Henriksson, M. Corcoran, C. Méndez-Vidal, K. G. Wiman, and M. Farnebo, “Wrap53, a natural p53 antisense transcript required for p53 induction upon DNA damage,” Molecular Cell, vol. 33, no. 4, pp. 462–471, 2009.
- M. M. Candeias, L. Malbert-Colas, D. J. Powell et al., “p53 mRNA controls p53 activity by managing Mdm2 functions,” Nature Cell Biology, vol. 10, no. 9, pp. 1098–1105, 2008.
- G. T. Bommer, I. Gerin, Y. Feng et al., “p53-mediated activation of miRNA34 candidate tumor-suppressor genes,” Current Biology, vol. 17, no. 15, pp. 1298–1307, 2007.
- L. He, X. He, L. P. Lim et al., “A microRNA component of the p53 tumour suppressor network,” Nature, vol. 447, no. 7148, pp. 1130–1134, 2007.
- V. Tarasov, P. Jung, B. Verdoodt et al., “Differential regulation of microRNAs by p53 revealed by massively parallel sequencing: miR-34a is a p53 target that induces apoptosis and G -arrest,” Cell Cycle, vol. 6, no. 13, pp. 1586–1593, 2007.
- T. C. Chang, E. A. Wentzel, O. A. Kent et al., “Transactivation of miR-34a by p53 broadly influences gene expression and promotes apoptosis,” Molecular Cell, vol. 26, no. 5, pp. 745–752, 2007.
- N. Raver-Shapira, E. Marciano, E. Meiri et al., “Transcriptional activation of miR-34a contributes to p53-mediated apoptosis,” Molecular Cell, vol. 26, no. 5, pp. 731–743, 2007.
- D. P. Bartel, “MicroRNAs: genomics, biogenesis, mechanism, and function,” Cell, vol. 116, no. 2, pp. 281–297, 2004.
- A. Eulalio, E. Huntzinger, and E. Izaurralde, “Getting to the root of miRNA-mediated gene silencing,” Cell, vol. 132, no. 1, pp. 9–14, 2008.
- D. Baek, J. Villén, C. Shin, F. D. Camargo, S. P. Gygi, and D. P. Bartel, “The impact of microRNAs on protein output,” Nature, vol. 455, no. 7209, pp. 64–71, 2008.
- M. Selbach, B. Schwanhäusser, N. Thierfelder, Z. Fang, R. Khanin, and N. Rajewsky, “Widespread changes in protein synthesis induced by microRNAs,” Nature, vol. 455, no. 7209, pp. 58–63, 2008.
- H. Guo, N. T. Ingolia, J. S. Weissman, and D. P. Bartel, “Mammalian microRNAs predominantly act to decrease target mRNA levels,” Nature, vol. 466, no. 7308, pp. 835–840, 2010.
- C. J. Braun, X. Zhang, I. Savelyeva et al., “p53-responsive microRNAs 192 and 215 are capable of inducing cell cycle arrest,” Cancer Research, vol. 68, no. 24, pp. 10094–10104, 2008.
- S. A. Georges, M. C. Biery, S. Y. Kim et al., “Coordinated regulation of cell cycle transcripts by p53-inducible microRNAs, miR-192 and miR-215,” Cancer Research, vol. 68, no. 24, pp. 10105–10112, 2008.
- L. Bohlig, M. Friedrich, and K. Engeland, “p53 activates the PANK1/miRNA-107 gene leading to downregulation of CDK6 and p130 cell cycle proteins,” Nucleic Acids Research, vol. 39, no. 2, pp. 440–453, 2011.
- Y. Takahashi, A. R. R. Forrest, E. Maeno, T. Hashimoto, C. O. Daub, and J. Yasuda, “MiR-107 and MiR-185 can induce cell cycle arrest in human non small cell lung cancer cell lines,” PLoS One, vol. 4, no. 8, Article ID e6677, 2009.
- M. Yamakuchi, C. D. Lotterman, C. Bao et al., “P53-induced microRNA-107 inhibits HIF-1 and tumor angiogenesis,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 14, pp. 6334–6339, 2010.
- R. Brosh, R. Shalgi, A. Liran et al., “p53-repressed miRNAs are involved with E2F in a feed-forward loop promoting proliferation,” Molecular Systems Biology, vol. 4, Article ID 229, 2008.
- M. Sachdeva, S. Zhu, F. Wu et al., “p53 represses c-Myc through induction of the tumor suppressor miR-145,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 9, pp. 3207–3212, 2009.
- H. L. Yan, G. Xue, Q. Mei et al., “Repression of the miR-17-92 cluster by p53 has an important function in hypoxia-induced apoptosis,” EMBO Journal, vol. 28, no. 18, pp. 2719–2732, 2009.
- M. Guttman, I. Amit, M. Garber et al., “Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals,” Nature, vol. 458, no. 7235, pp. 223–227, 2009.
- A. M. Khalil, M. Guttman, M. Huarte et al., “Many human large intergenic noncoding RNAs associate with chromatin-modifying complexes and affect gene expression,” Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 28, pp. 11667–11672, 2009.
- M. Huarte, M. Guttman, D. Feldser et al., “A large intergenic noncoding RNA induced by p53 mediates global gene repression in the p53 response,” Cell, vol. 142, no. 3, pp. 409–419, 2010.
- B. Wang, Z. Xiao, H. L. Ko, and E. C. Ren, “The p53 response element and transcriptional repression,” Cell Cycle, vol. 9, no. 5, pp. 870–879, 2010.
- F. Murray-Zmijewski, E. A. Slee, and X. Lu, “A complex barcode underlies the heterogeneous response of p53 to stress,” Nature Reviews Molecular Cell Biology, vol. 9, no. 9, pp. 702–712, 2008.
- N. Moskovits, A. Kalinkovich, J. Bar, T. Lapidot, and M. Oren, “p53 attenuates cancer cell migration and invasion through repression of SDF-1/CXCL12 expression in stromal fibroblasts,” Cancer Research, vol. 66, no. 22, pp. 10671–10676, 2006.
- J. Pesch, U. Brehm, C. Staib, and F. Grummt, “Repression of interleukin-2 and interleukin-4 promoters by tumor suppressor protein p53,” Journal of Interferon and Cytokine Research, vol. 16, no. 8, pp. 595–600, 1996.
- U. Santhanam, A. Ray, and P. B. Sehgal, “Repression of the interleukin 6 gene promoter by p53 and the retinoblastoma susceptibility gene product,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 17, pp. 7605–7609, 1991.
- J. Ahn, M. Murphy, S. Kratowicz, A. Wang, A. J. Levine, and D. L. George, “Down-regulation of the stathmin/Op18 and FKBP25 genes following p53 induction,” Oncogene, vol. 18, no. 43, pp. 5954–5958, 1999.
- W. E. Mercer, M. T. Shields, D. Lin, E. Appella, and S. J. Ullrich, “Growth suppression induced by wild-type p53 protein is accompanied by selective down-regulation of proliferating-cell nuclear antigen expression,” Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 5, pp. 1958–1962, 1991.
- L. C. Harris, J. S. Remack, P. J. Houghton, and T. P. Brent, “Wild-type p53 suppresses transcription of the human O6-methylguanine-DNA methyltransferase gene,” Cancer Research, vol. 56, no. 9, pp. 2029–2032, 1996.
- V. Iotsova, P. Crépieux, C. Montpellier, V. Laudet, and D. Stehelin, “TATA-less promoters of some Ets-family genes are efficiently repressed by wild-type p53,” Oncogene, vol. 13, no. 11, pp. 2331–2337, 1996.
- K. Rother, Y. Y. Li, K. Tschöp et al., “Expression of cyclin-dependent kinase subunit 1 (Cks1) is regulated during the cell cycle by a CDE/CHR tandem element and is downregulated by p53 but not by p63 or p73,” Cell Cycle, vol. 6, no. 7, pp. 853–862, 2007.
- K. Rother, M. Dengl, J. Lorenz et al., “Gene expression of cyclin-dependent kinase subunit Cks2 is repressed by the tumor suppressor p53 but not by the related proteins p63 or p73,” FEBS Letters, vol. 581, no. 6, pp. 1166–1172, 2007.
- P. Arizti, L. Fang, I. Park et al., “Tumor suppressor p53 is required to modulate BRCA1 expression,” Molecular and Cellular Biology, vol. 20, no. 20, pp. 7450–7459, 2000.
- T. K. MacLachlan, B. C. Dash, D. T. Dicker, and W. S. El-Deiry, “Repression of BRCA1 through a feedback loop involving p53,” Journal of Biological Chemistry, vol. 275, no. 41, pp. 31869–31875, 2000.
- B. Singh, P. G. Reddy, A. Goberdhan et al., “p53 regulates cell survival by inhibiting PIK3CA in squamous cell carcinomas,” Genes and Development, vol. 16, no. 8, pp. 984–993, 2002.
- S. Sengupta, A. Shimamoto, M. Koshiji et al., “Tumor suppressor p53 represses transcription of RECQ4 helicase,” Oncogene, vol. 24, no. 10, pp. 1738–1748, 2005.
- T. Criswell, D. Klokov, M. Beman, J. P. Lavik, and D. A. Boothman, “Repression of IR-inducible clusterin expression by the p53 tumor suppressor protein,” Cancer Biology and Therapy, vol. 2, no. 4, pp. 372–380, 2003.
- X. Luo, Q. He, Y. Huang, and M. S. Sheikh, “Cloning and characterization of a p53 and DNA damage down-regulated gene PIQ that codes for a novel calmodulin-binding IQ motif protein and is up-regulated in gastrointestinal cancers,” Cancer Research, vol. 65, no. 23, pp. 10725–10733, 2005.
- S. Sohr and K. Engeland, “RHAMM is differentially expressed in the cell cycle and downregulated by the tumor suppressor p53,” Cell Cycle, vol. 7, no. 21, pp. 3448–3460, 2008.