468705.fig.002a
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468705.fig.002b
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468705.fig.002c
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468705.fig.002d
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468705.fig.002e
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468705.fig.002f
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468705.fig.002g
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468705.fig.002h
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Figure 2: In vitro neurotrophic effects of ATSC. LAN-5 human neuroblasts and PC12 rat cells were cultured either ATSC-conditioned medium or co-cultured with ATSC and morphological modifications were monitored over time: (a) LAN5 in standard culture medium; (b) LAN5 cultured in ATSC-CM for 72 hours; (c) and (d) LAN-5 co-cultured with ATSC using a cell density of 104 cell/cm2 for both cell populations; (e) PC12 in standard culture medium; (f) PC12 cultured in ATSC-CM for 4 days; (g) PC12 cultured in βNGF 100 ng/mL for 4 days; (h) PC12 cultured in HDF-conditioned medium for 4 days. Arrows show evidence of neurite outgrowth; asterisk (*) indicate ATSC in culture. Scale bar 100 μm in all panels except for panel μm.