Neurotrophic Features of Human Adipose Tissue-Derived Stromal Cells: In Vitro and In Vivo Studies

Figure 2

In vitro neurotrophic effects of ATSC. LAN-5 human neuroblasts and PC12 rat cells were cultured either ATSC-conditioned medium or co-cultured with ATSC and morphological modifications were monitored over time: (a) LAN5 in standard culture medium; (b) LAN5 cultured in ATSC-CM for 72 hours; (c) and (d) LAN-5 co-cultured with ATSC using a cell density of 10⁴ cell/cm² for both cell populations; (e) PC12 in standard culture medium; (f) PC12 cultured in ATSC-CM for 4 days; (g) PC12 cultured in βNGF 100 ng/mL for 4 days; (h) PC12 cultured in HDF-conditioned medium for 4 days. Arrows show evidence of neurite outgrowth; asterisk (*) indicate ATSC in culture. Scale bar 100 μm in all panels except for panel 𝑑=10 μm.

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