Figure 4: SEM-Tanaka sequence of intravacuolar proliferation of tachyzoites. Arrowheads indicate extensions of the MNN, which connect the incurved face of a single parasite to the PVM (1 h), the incurved faces of parasites after the first and second divisions (6, 12 h), or which connect the rosette to the PVM (24 h). Arrows point out the RB (6–24 h). Asterisks indicate the apparent empty space at the PV periphery (1–24 h). Timing indicated in lower left corners represents the replication time after invasion. μm.