Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing
Figure 3
Neuron-specific gene expression of hMSCs during neural differentiation. (a) Cells that had been harvested 6 hr for Nurr-1, 12 hr for NeuroD6, and 48 hr for NF-L and NF-M were used in RT-PCR assay. NGM: normal growth media; NIM: neural induction media; NDM: neural differentiation media. (b) The relative expression of Nurr-1, NF-L, and NF-M was normalized by GAPDH using densitometric analysis. Data for NeuroD6 were omitted because bands corresponding to NeuroD6 were too faint to be measured by densitometry.
