Thin Filament-Reconstituted Skinned Muscle Fibers for the Study of Muscle Physiology

Figure 1

Schematic diagram illustrating the thin filament removal and reconstitution protocol. By applying gelsolin to skinned muscle fibers (a), thin filament free muscle fibers (b) can be obtained. By adding actin monomer to these thin filament free muscle fibers, actin-filament-reconstituted muscle fibers without regulatory proteins (c) can be obtained. Thin filament can be reconstituted by adding regulatory proteins to these actin-filament-reconstituted muscle fibers.