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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 492075, 9 pages
http://dx.doi.org/10.1155/2011/492075
Research Article

Localization of Magic-F1 Transgene, Involved in Muscular Hypertrophy, during Early Myogenesis

1Human Anatomy Section, University of Pavia, Via Forlanini 8, 27100 Pavia, Italy
2Center for Tissue Engineering (CIT), University of Pavia, 27100 Pavia, Italy
3Translational Cardiomyology (SCIL), Katholieke Universiteit Leuven, Herestraat 49, 3000 Leuven, Belgium
4Institute of Molecular Genetics, CNR, 27100 Pavia, Italy
5Stem Cell Research Institute, University Hospital Gasthuisberg, Herestraat 49, 3000 Leuven, Belgium

Received 31 May 2011; Revised 14 September 2011; Accepted 19 September 2011

Academic Editor: Aikaterini Kontrogianni-Konstantopoulos

Copyright © 2011 Flavio Ronzoni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We recently showed that Magic-F1 (Met-activating genetically improved chimeric factor 1), a human recombinant protein derived from hepatocyte growth factor/scatter factor (HGF/SF) induces muscle cell hypertrophy but not progenitor cell proliferation, both in vitro and in vivo. Here, we examined the temporal and spatial expression pattern of Magic-F1 in comparison with Pax3 (paired box gene 3) transcription factor during embryogenesis. Ranging from 9.5 to 17.5 dpc (days post coitum) mouse embryos were analyzed by in situ hybridization using whole mounts during early stages of development (9.5–10.5–11.5 dpc) and cryostat sections for later stages (11.5–13.5–15.5–17.5 dpc). We found that Magic-F1 is expressed in developing organs and tissues of mesenchymal origin, where Pax3 signal appears to be downregulated respect to the wt embryos. These data suggest that Magic-F1 could be responsible of muscular hypertrophy, cooperating with Pax3 signal pathway in skeletal muscle precursor cells.