492075.fig.003
Figure 3: Transcription expression profile of Pax3 (a–c) and Magic-F1 (d–f) on 11.5 dpc mouse embryos of hemizygous Magic-F1 (a–f) and wt (g, h) mice by in situ hybridization. In the sagittal section of transgenic embryo, Pax3 riboprobes reveal a strong signal at the level of hindbrain (arrowhead in a), neuroepithelium of neural tube (arrow in b), and dorsal somites (c); sagittal section of 11.5 dpc embryo hybridized by Magic-F1 riboprobes (d) shows a similar pattern of Pax3 although no expression was detected at the level of neuroepithelium of neural tube (arrow in e); specific transcripts are observed in the cervical dorsal (posterior) root ganglion (arrows in c and f) and somites (arrowheads in f). In 11.5 dpc wt embryo hybridized with Pax3 riboprobes (g), specific transcripts are detected mostly in the cerebellar primordium (cerebellar rudiment derived from dorsal part of the alar lamina of the Metencephalon) (arrow) and somites in lumbar region (arrowhead in g), whereas no signal for Magic-F1 has been detected (h). Immunofluorescence analysis confirmed the presence of recombinant protein at the level of thoracic (i) and lumbar (j) somites in the transgenic embryos, whereas the signal was faint in the wt embryos (k), probably referred only to the HGF expression at this stage.