497364.fig.002a
(a)
497364.fig.002b
(b)
Figure 2: Presentation of WSN-OVAI/II virus by dendritic cells (DCs) in vitro to OVA-specific CD8+ and CD4+ T cells. (a) Splenic DCs were incubated with either live or heat-inactivated (HI) WSN-OVAI/II virus and then washed and titrated in microtiter plates. OT-I cells and OT-II cells were added to the cultures (105/well), and T cell proliferation was estimated by use of [3H]thymidine incorporation two days later. The data shown are the means (±SEM) from triplicate cultures. (b) CFSE-labelled naïve OT-I and OT-II cells were coincubated with live virus-pulsed or control DCs. Three days later, proliferation of CD8+ and CD4+ T cells was determined by using flow cytometry. The results shown are representative of three experiments.