Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells
Figure 2
Staining for SAA is found in the nucleus, nanotubes, and budding vesicles; however, its association with endomembranes remains unclear. SAA in HCAEC shows a predominantly nuclear localization, with a strong signal also concentrated in the juxta-nuclear cytoplasm (a′). In (a) and (a′) the cut views above and on the right of each panel represents the SAA mainly inside nuclei and in the perinuclear space. In HCAEC nuclei were stained with DAPI (blue), endomembranes were stained with 3,3′-dihexyloxacarbocyanin iodide (DiO6) (green) and SAA was labeled with anti-SAA antibodies (red) (c). Merged labeling indicates SAA staining distinct from the endomembranes (b) and (c). Endoplasmic reticulum is shown by the purple arrows, mitochondrion with the green arrow. HCAEC were stained with DiO6 and show colocalization of SAA with nanotubes (d). SAA was clearly detected at the tips of the filopodial protrusions (white arrows), and in vesicles dispersed outside the cells (white arrowheads). The association of SAA with endomembranes remains unclear. Anti-SAA polyclonal antibodies 3, 5, and 6, which target SAA peptides with amino acid sequences 27–44, 59–72, and 68–84, respectively, were used in the designated SAA panels.
