Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells

Figure 3

Multimerization of SAA. Human recombinant SAA was blotted onto nitrocellulose membranes and overlayed with polyclonal antibodies against synthetic SAA human peptides ranging from the indicated amino acid sequences: lane 1: SAA1–17, lane 2: SAA14–30, lane 3: SAA27–44, lane 4: SAA40–63, lane 5: SAA59–72, lane 6: SAA68–84, lane 7: SAA79–94, and lane 8: SAA 89–104. HRP-labeled secondary goat antirabbit antibody was used for secondary incubation followed by luminol and chemiluminescent detection. The SAA monomer at around 12 kD is indicated.