Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination

Figure 2

Insert size determination of orangutan BAC library clones. DNA from 92 random BAC clones was isolated, digested with NotI and separated using pulse field gel electrophoresis (PFGE) on two gels. Low Range PFG marker (NEB) was loaded in the flanking wells. A subset of the analyzed BACs from a single gel is shown. Forty-one recombinant clones and 2 noninsert clones can be seen. The band comigrating in all clones with the 9-Kb marker band is the BAC vector.