Research Article

Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination

Table 1

Selectivity of recombination from BAC pools in DY380 cells using 100 ng of linear recombination cassette.

Complexity of BAC poolNo. of recombinant clones

14,800–10,000
1/10450–1,000
1/10055–100
1/1,0008–12
1/10,0001-2