Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma
Figure 1
Comparison of AGP purified by solely chromatographic Method 1 or hot phenol-incorporating Method 2. (a) shows Coomassie-stained SDS reducing gels of equivalent (5 μg total protein/lane) samples of AGP preparations after initial (Cibacron Blue dye column flow-through, Affi-Blue FT, or hot phenol aqueous phase, Phenol sup.) or secondary hydroxyapatite chromatography (HT), with the Method number given beneath, and the position of molecular mass markers indicated between (in kDa) the gel images. (b) shows the percentage of the injected dose remaining in mice following injection of radioiodinated AGP purified by either Method 1 or Method 2 (, mean ± SD) versus time. (c) is identical to (b), except that the first hour post-injection is expanded to allow visualization of initial time points.
