Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma
Figure 2
Reaction of AGP with neuraminidase and reaction of Method 1 versus Method 2 AGP with lectins and antibodies. AGP purified by Method 2 (AGP; 2 μg total protein/lane) was treated with or without neuraminidase (neu-AGP) and examined on SDS reducing gels or replica SNA blots (a). Molecular mass markers (in kDa) are shown at left. (b) shows 0.25–1.0 μg AGP from Methods 1 or 2 on blots probed either with Sambucus nigra agglutinin (SNA Blot) or with Anti-AGP antibodies. The SNA: anti-AGP ratio of the band intensities is shown beneath the lanes.
