Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma
Figure 3
Comparison of AGP purified by solely chromatographic Method 3 or sulphosalicylic acid-incorporating Method 4. (a) and (b) show Coomassie-stained SDS reducing gels of equivalent (5 μg total protein/lane) samples of AGP preparations at intermediate steps of either Method of AGP purification: Affi-Blue FT, Cibacron Blue dye column flow-through, CHT, ceramic hydroxyapatite peak; HT Affi-Blue, HiTrap Cibacron Blue dye column flow-through; SSA Sup., supernatant following sulphosalicylic acid precipitation and neutralization. (c) depicts nitrocellulose blots of replicated gels containing 1.0 μg of purified human serum albumin (HSA) or of AGP purified by either Method 3 (M3) or Method 4 (M4) and probed either with Anti-AGP antibodies (at left) or Sambucus nigra agglutinin (SNA lectin) at left. The position of molecular mass markers and their mass in kDa is shown at left of each panel.
