Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma
Figure 4
In vivo clearance, circular dichroism spectra, and deglycosylation of AGP purified by Methods 3 or 4. (a) shows residual, normalized acid-precipitable radioactivity in rabbit plasma following injection of radioiodinated AGP purified as indicated by arrowed text. (b) depicts circular dichroism spectra of AGP purified by Method 3 (upper) and Method 4 (lower); Δε represents the difference between the absorption by AGP of left-handed and right-handed circularly polarized light at different wavelengths. (c) shows samples of AGP purified by either method, with (+) or without (−) reaction with PNGase F; reaction of commercial AGP (Sigma) is also shown. Molecular mass markers are shown at left in kDa.
