Comparison of Methods for the Purification of Alpha-1 Acid Glycoprotein from Human Plasma

Table 1

Comparison of stepwise purity and yield of AGP purification methods.

	Total protein (mg)	AGP (mg)	AGP/total protein	Fold enrichment versus starting material
Method 3
Plasma	1786	17.1	0.0096	1.0
Affi-Gel Blue FT	86.6	17.8	0.21	21
CHT Eluate	16.3	9.1	0.56	58
HiTrap Blue FT	8.7	9.3	1.06	110
Method 4
Plasma	1786	17.7	0.0099	1.0
SSA Supernatant	43.3	13.3	0.31	31
CHT Eluate	20.8	7.8	0.38	38
HiTrap Blue FT	9.19	8.1	0.89	89