587470.fig.002a
(a)
587470.fig.002b
(b)
587470.fig.002c
(c)
587470.fig.002d
(d)
Figure 2: Multiphoton images of Caco-2 cells treated with PEG 8000 and various bacterial strains. Uninfected cells were served as control (a). Caco-2 cells were infected with commensal strains, 261 and HS4 (b, c), and EPEC (d). The maximum depth of invasion was seen in scaffolds infected with HS4. The overall thickness of the scaffold was approximately 400 μm. The actin filaments are shown in red and the DAPI staining of nuclei are shown in blue. Bar length = 10 μm.