Staining of neutral lipid droplets (LDs) with BODIPY (493/503) in cross section of rat soleus muscle demonstrates staining within the fiber, but especially under the surrounding sarcolemma. (a) Fibers stained with BODIPY (green) demonstrate the appearance of spherical LDs within the muscle fibers as previously described with Oil Red O staining procedures. (b) To provide anatomical localization, the membrane is labeled with wheat germ agglutinin (WGA)-Alexa Fluor 594 (red). (c) Nuclei were stained with DAPI (Blue). (d) Merged triple label image of the muscle section. All images were taken at 63x, and a scale bar is provided for reference.